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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (1): 212-217.doi: 10.11924/j.issn.1000-6850.2011-2735

• 林学 园艺 园林 • 上一篇    下一篇

福林酚试剂法和紫外分光光度法测定冬枣多酚含量的比较研究

卜彦花 周娜娜 王春悦 曹建康 赵玉梅   

  • 收稿日期:2011-09-19 修回日期:2011-10-30 出版日期:2012-01-05 发布日期:2012-01-05
  • 基金资助:

    国家自然科学基金项目

Comparative Study on Determination of Total Phenolics Content of Jujube Fruit with FC and UV-spectrophotometric Method

  • Received:2011-09-19 Revised:2011-10-30 Online:2012-01-05 Published:2012-01-05

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摘要：

为了提高果实多酚的提取率和评价目前多酚含量测定方法的可比性，利用福林酚试剂法和紫外分光光度法测定冬枣果实多酚含量，并研究了不同浓度的甲醇-水、乙醇-水提取体系对2种方法测定结果的影响。试验表明，紫外分光光度测定法的线性范围为1375~12500 mg/kg，福林酚试剂测定法的线性范围为7500~15000 mg/kg。紫外分光光度测定法的检出限和定量限都显著低于福林酚法测定法。通过测定回收率，发现这2种测定方法都具有良好的精密度和准确度。冬枣多酚适宜提取体系为60%~80%（v/v）甲醇-水或乙醇-水的复合体系，且前者的提取率显著（P<0.001）高于后者。利用福林酚试剂法测定的冬枣多酚含量明显高于紫外分光光度法，两者的关系为Y（福林酚试剂法）=1.538x+1221（紫外分光光度法），r=0.956。研究结果为果实多酚的提取、测定研究提供了理论指导和参考方法。

关键词: 污染防治, 污染防治

Abstract:

In order to improve the extraction yield of fruit phenolics and evaluate the comparability of total phenolics content determination method, total phenolics content of jujube fruit was comparatively determined by using Folin Ciocalteu (FC) and UV-spectrophotometric methods, respectively. Meanwhile, the influence of different extraction systems on the testing results from the two methods was studied. Results from this study indicated that linear range of UV-spectrophotometric method was 1375-12500 mg/kg, linear range of FC method was 7500-15000 mg/kg. The detection limit and the quantifying limit of UV-spectrophotometric method were significantly lower than those of FC method. Precision and accuracy of the two methods were acceptable by detecting the recovery rate. The results also showed that appropriate solution for extracting total phenolics from jujube fruit was 60%-80% (v/v) methanol-water or ethanol-water system. Moreover, the extraction yield of the former was significantly (P<0.001) higher than the latter. Total phenolics content of jujube fruit determined by FC was significantly higher than that determined by UV-spectrophotometric method. The linear regression was: Y (FC) =1.538x+1221 (UV-spectrophotometric), r=0.956. The results would provide the theoretical guidance and reference methods for the research of total phenolics in fruits and vegetables.

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