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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 121-126.doi: 10.11924/j.issn.1000-6850.2011-2901

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

大白菜抗芜菁花叶病毒(TuMV)EST-SSR信息分析与引物筛选

张晓亮 张志刚 赵智中 宋希云 李巧云   

  • 收稿日期:2011-10-09 修回日期:2011-12-20 出版日期:2012-04-05 发布日期:2012-04-05

AnalEST-SSR Analysis and Primer Screening of Turnip Mosaic Virus (TuMV) Resistance in Chinese Cabbage

  • Received:2011-10-09 Revised:2011-12-20 Online:2012-04-05 Published:2012-04-05

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摘要：

为了研究大白菜抗芜菁花叶病毒(TuMV)，对大白菜及芸薹属中与抗性有关的EST序列进行了搜索和SSR筛选，并根据SSR两端的序列设计引物，以1对大白菜TuMV抗、感材料的基因组DNA为模板，对上述引物进行了筛选。结果表明，在132条大白菜EST和895条芸薹属抗性EST序列中，共筛选出166个符合条件的SSR(16.2%)。其中包含6种核苷酸重复基元，占主导地位的分别是二核苷酸重复(33.7%)和三核苷酸重复(50.0%)，其余重复类型所占比例较小。根据上述SSR两翼的序列，共设计引物196对。引物筛选结果表明，能够进行成功扩增的有156对，占79.6%，其中35对引物在2份材料中具有多态性。因此，利用芸薹属中抗性有关的EST序列设计SSR引物，可用于大白菜TuMV抗性有关基因的标记。

关键词: 土地集约利用, 土地集约利用, 相对效率评价, 数据包络分析, 投影分析, 长沙市

Abstract:

In order to study turnip mosaic virus (TuMV) in Chinese cabbage, ESTs in Chinese cabbage and resistant ESTs in Brassica were searched and SSRs were screened. Primers were designed according to the sides sequence and were screened using the genome DNA of one pair of TuMV resistant and susceptible materials of Chinese cabbage as templates. The results showed that a total of 166 (16.2%) SSRs were screened from 132 Chinese cabbage ESTs and 895 resistant ESTs of Brassica, among which there were six kinds of nucleotide repeat motifs, the dinucleotide (33.7%) and trinucleotide (50.0%) repeats were the main types, proportions for other repeat types were much small. 196 primers were designed according to the sides sequence of the above SSRs, among which 156 (79.6%) were amplified successfully, and 35 primers were amplified polymorphic in the two materials. Therefore, SSR primers designed from Brassica resistant EST sequences could be used in marking of TuMV resistant genes of Chinese cabbage.