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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (15): 36-41.doi: 10.11924/j.issn.1000-6850.2011-3123

所属专题： 棉花

• 农学 农业基础科学 • 上一篇    下一篇

棉花区试品种的AFLP指纹图谱分析

孙宁 付小琼 王秀玲 刘逢举 耿向阳 曲京武 乔铁军 杨付新   

  • 收稿日期:2011-10-26 修回日期:2011-12-20 出版日期:2012-05-25 发布日期:2012-05-25
  • 基金资助:

    公益性院所长基金

AFLP Fingerprinting Analysis of 31 Cultivars Attending the National Regional Trial

  • Received:2011-10-26 Revised:2011-12-20 Online:2012-05-25 Published:2012-05-25

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摘要：

为了探索AFLP分子标记在构建国家区试棉花品种指纹图谱上的优势，以2009年和2010年参加国家黄河流域棉花区试的B组常规棉、C组杂交棉共31份品种为材料，运用筛选确定的2对AFLP核心引物对这些品种进行分子标记分析，共获得44个多态性片段，其中多态性位点占总扩增位点的28.39%，利用44个多态性片段初步构建了这些品种的AFLP指纹图谱。聚类结果显示，在相似系数为0.92时，31份区试品种完全区分开；常规棉之间、杂交棉之间大多不同程度的优先聚集在一起。

关键词: 新疆农业科学院土壤肥料与农业节水研究所, 新疆农业科学院土壤肥料与农业节水研究所, 乌鲁木齐830091

Abstract:

For exploring the advantage of AFLP molecular marker in constructing the cotton fingerprinting, 31 cultivars of conventional cottons in team B, hybrid cottons in team C attending the 2009 and 2010 national regional trials were used for DNA fingerprinting. Two pairs of core AFLP primer were used for polymorphism analysis. Forty-four polymorphic bands were obtained which took 28.39% of total amplified bands. A primary DNA fingerprint database was established with 44 polymorphic bands. The cluster analysis showed that, all tested-cultivars could be completely identified at 0.92 similarity index level, and conventional cottons almost cluster together preferentially in different extent, so as hybrid cottons.