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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 213-218.doi: 10.11924/j.issn.1000-6850.2011-3236

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

灵芝群体交配基因型分析

陈裕新 夏志兰 刘鹏 康信聪 洪亚辉 刘东波   

  • 收稿日期:2011-11-03 修回日期:2012-01-29 出版日期:2012-04-05 发布日期:2012-04-05
  • 基金资助:

    国家科技支撑计划“中药产业区域发展及特色产品研究开发”;科技部科技人员服务企业行动计划“珍稀食用菌系列产品开发”

Mating Genotype Analysis of Ganoderma lucidum Populations

  • Received:2011-11-03 Revised:2012-01-29 Online:2012-04-05 Published:2012-04-05

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摘要：

研究灵芝群体交配基因型，并与酯酶同工酶试验相比较，探讨它们在群体亲缘关系分析上的异同点。采用OWE-SOJ技术对24个灵芝菌株的单孢分离菌株进行标准交配型鉴定，及群体间交配基因型的测定，并结合酯酶同工酶试验对群体间的亲缘关系进行分析。鉴定出7大类交配基因型，并发现A因子含有4个等位基因，B因子含有4个等位基因，及一个特殊的A混合基因，四类交配型出现了一定程度的偏分离。酯酶同工酶试验检测出了28条不同酯酶酶谱带，在相似系数为0.73214时，样品被分为9大类。比较交配基因型分析与酯酶同工酶试验结果，发现两者具有很高的相似性。交配基因型测定可以作为分析菌株间差异和鉴定品种的一种重要补充手段。

关键词: 发展对策, 发展对策

Abstract:

The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. The results showed that all strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of a factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mating types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isoenzyme test. Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing mating genotype analysis with esterase isozyme analysis, it showed great similarity. Mating genotype analysis could be an important supplementary method to strains identification and genetic diversity research.