关键词: 营养诊断, 营养诊断

Abstract:

In order to investigate the difference among different sterile lines of Tagetes erecta, the pollen viability and stigma receptivity of it was studied in this paper. The pollen germination in vitro was used to select the optimum geminating temperature of the pollen, to observe daily variation of pollen viability and to choose optimum storage condition of marigold ‘V-01’ (Tagetes erecta L.) pollen. The stigma receptivity was tested with benzidine-H2O2 method. Aniline blue water soluble staining was for detecting the pollen germination on stigma. The results showed that: (1) 25-30℃ was the best temperature for the pollen germination. The pollen viability increased firstly in the morning, reached the maximum at 11 am to 13 pm and then decreased. 4℃ with dry condition was the best pollen storage condition. (2) Stigma with the shape of ‘γ’ was respectable and could sustain 3 days. (3)Pollen geminated on stigma 1 hour later and pollen cells flew into the pollen tube 2 hours later after the pollination.