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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (36): 92-98.doi: 10.11924/j.issn.1000-6850.casb15090014

所属专题： 园艺

• 农学 农业基础科学 • 上一篇    下一篇

采用SPAD仪进行甜菜氮素营养诊断技术研究

王秋红1,2,周建朝1,2,王孝纯1,2   

  1. (1黑龙江大学农作物研究院,哈尔滨 150080；2中国农业科学院甜菜研究所,哈尔滨 150080）
  • 收稿日期:2015-09-06 修回日期:2015-10-13 接受日期:2015-10-23 出版日期:2015-12-30 发布日期:2015-12-30
  • 通讯作者: 周建朝
  • 基金资助:
    农业部现代农业产业技术体系建设项目“国家甜菜现代农业产业技术体系土壤肥料岗位”(CARS-210306)；国家自然科学基金项目“不同基因型甜菜根际土壤有机氮矿化特征及影响机理”(31371686)。

Diagnostic Technique of Sugarbeet Nitrogen Nutrient Status Using SPAD-502 Chlorophyll Meter

Wang Qiuhong1,2, Zhou Jianchao1,2, Wang Xiaochun1,2   

  1. (1Crop Academy of Heilongjiang University, Harbin 150080;2Sugarbeet Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150080)
  • Received:2015-09-06 Revised:2015-10-13 Accepted:2015-10-23 Online:2015-12-30 Published:2015-12-30

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摘要： 为研究甜菜大田栽培及生产过程中氮素的营养状态，以便及时补充氮素亏缺，使用SPAD-502仪(soil plant analysis development)测定了甜菜叶片不同叶位和叶序的SPAD值，发现SPAD值的分布特点及其规律性，建立了基于SPAD值的氮素营养快速诊断方法。试验设计了室内盆栽及田间试验，对不同基因型、不同发育阶段甜菜叶片不同叶位叶序的SPAD值进行测定，统计并分析SPAD值与叶片的叶绿素含量及植株全氮含量的相关性。结果表明：甜菜叶片SPAD值在不同叶位及叶序上有差异，其中叶片尖部SPAD最大值出现的次数与叶片侧缘部和叶片基部相比较有较明显优势，施氮及不施氮处理均达到50%以上；4~6片真叶期后最高叶片SPAD值与测得的同株叶片的SPAD值有较显著的相关性，所以最高叶片的叶片尖部可作为甜菜叶片SPAD值的最适测定部位；增加施氮量能提高叶片SPAD值；不同品种间叶片SPAD值也有差异；SPAD值与不同发育阶段甜菜叶片的叶绿素含量以及植株全氮含量分别建立线性方程，拟合后发现叶片SPAD值和总叶绿素含量之间为极显著相关，与植株含氮量之间为相关性显著。因此，利用叶片SPAD值可实时监测甜菜生长与光合效应，进行氮素营养的快速诊断。

关键词: 秸秆还田深度, 秸秆还田深度, 小麦, 蔗糖, 产量

Abstract: In field planting and production of sugarbeet, nitrogen nutrient should be supplied in time. The distribution feature of SPAD value on different leaf positions and leaf orders of sugarbeet was analyzed using SPAD-502 chlorophyll meter so that the rapid diagnostic technique of sugarbeet nitrogen nutrient status could be built on the SPAD value. Both pot and field experiment with different sugarbeet genotypes were conducted in order to measure the leaf SPAD value of different development stages in different genotypes of sugarbeet. Then, the authors counted and analyzed the correlations between the leaves’ SPAD value and its chlorophyll content or the plant nitrogen content in different genotypes of sugarbeet. The results were as follows: the leaves’ SPAD values of sugarbeet were different among different leaf positions and leaf orders. The repeated times of the biggest SPAD value of leaf tip were obviously more than that of the margin and lower part of the leaf, both treatments (N150 and N0) were all mixed up to 50%. The SPAD values of N150 were higher than those of N0, even they varied among different cultivars under each treatment. After 4-6 euphylla period of sugarbeet, the SPAD value of the highest leaf had a significant correlation with that of other leaves in the same plant, so the highest leaf tip of sugarbeet should be taken as the measured position of SPAD value. With high nitrogen application rates, the SPAD value increased. The SPAD values of different cultivars varied under each treatment. The linear equations were built respectively between SPAD values and chlorophyll concentration and plant total nitrogen content. After fitting the linear equations, the authors found that the SPAD value of leaf and total chlorophyll content of plant in different developmental stages (seedling, cluster rapid growth period and root sugar accumulation period) had a very significant correlation, the relationship between SPAD value of leaf and nitrogen content of plant was significant. So the development and photosynthetic efficiency of sugarbeet could be constantly monitored by SPAD value, and SPAD values of sugarbeet were suitable for rapid diagnosis of nitrogen nutrition.

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