咯菌腈与戊唑醇复配对小麦赤霉病菌的增效作用研究

doi:10.11924/j.issn.1000-6850.casb2021-0340

中国农学通报 ›› 2022, Vol. 38 ›› Issue (6): 116-120.doi: 10.11924/j.issn.1000-6850.casb2021-0340

所属专题：生物技术；植物保护；小麦

咯菌腈与戊唑醇复配对小麦赤霉病菌的增效作用研究

姜佳, 陈金鹏, 魏江桥, 郭旭昊, 车志平, 田月娥, 陈根强, 刘圣明()

河南科技大学园艺与植物保护学院植物保护系,河南洛阳 471023

收稿日期:2021-04-01 修回日期:2021-07-08 出版日期:2022-02-25 发布日期:2022-03-16
通讯作者: 刘圣明
作者简介:姜佳,女,1983年出生,河南安阳人,讲师,研究方向:杀菌剂毒理及抗药性。通信地址:471023 河南省洛阳市洛龙区开元大道263号河南科技大学杀菌剂生物学实验室,Tel:0379-64389603,E-mail: jiangjiazb@163.com。
基金资助:
河南省杰出青年科学基金“杀菌剂对禾谷镰孢菌DON毒素合成的影响及其调控机制研究”(212300410015);中原青年拔尖人才“抗药性禾谷镰孢菌生物学功能研究”(ZYQR201912157);河南省重大科技专项“小麦条锈病等重大病虫害绿色防控技术研究与应用”(201300111600);河南省高校科技创新人才支持计划“禾谷镰孢菌DON合成调控机制研究”(20HASTIT033);河南省高校青年骨干教师培养计划“禾谷镰孢菌抗多菌灵β₂-微管蛋白基因与DON毒素合成关系研究”(2018GGJS051)

Synergistic Effect of Fludioxonil and Tebuconazole Against Fusarium graminearum

JIANG Jia, CHEN Jinpeng, WEI Jiangqiao, GUO Xuhao, CHE Zhiping, TIAN Yue’e, CHEN Genqiang, LIU Shengming()

Department of Plant Protection, College of Horticultural and Plant Protection, Henan University of Science and Technology, Luoyang, Henan 471023

Received:2021-04-01 Revised:2021-07-08 Online:2022-02-25 Published:2022-03-16
Contact: LIU Shengming

摘要/Abstract

摘要：

研究旨在明确咯菌腈和戊唑醇复配剂抑制小麦赤霉病菌菌丝生长的最佳药剂配比。采用菌丝生长速率法测定了咯菌腈、戊唑醇及其复配剂对小麦赤霉病菌的有效抑制中浓度(EC₅₀)。采用wadley的增效比率法,评价了复配剂的增效作用。结果显示,咯菌腈和戊唑醇对小麦赤霉病菌的EC₅₀值分别为0.0205 μg/mL和0.1704 μg/mL;复配剂咯菌腈:戊唑醇的质量比在40:1,20:1,10:1,5:1,1:1,1:5,1:40时,SR值为0.54~1.28,呈相加作用;复配剂的质量比在1:10,1:20时,SR分别为1.56和1.62,呈增效作用。咯菌腈:戊唑醇的质量比在1:20时,SR值最大,为最佳增效作用比例。本试验为小麦赤霉病的防治及延缓戊唑醇的抗性产生提供了理论依据。

关键词: 咯菌腈, 戊唑醇, 小麦赤霉病, 禾谷镰孢菌, 增效作用

Abstract:

The purpose of this study is to determine the highest synergy ratio (SR) of the mixture of fungicide fludioxonil and tebuconazole that inhibited the mycelial growth of Fusarium graminearum. The effective concentration of 50% (EC₅₀) of fludioxonil, tebuconazole and their mixtures against F. graminearum isolates were determined by mycelium growth rate method. The SR of the mixture of the fungicide fludioxonil and tebuconazole was determined by Wadley’s SR method. The synergistic ratio of the mixture of the fungicide fludioxonil and tebuconazole was evaluated. The results showed that the EC₅₀ value of fludioxonil and tebuconazole against F. graminearum was 0.0205 μg/mL and 0.1704 μg/mL, respectively. When the mass ratio of the mixture of fludioxonil and tebuconazole was 40:1, 20:1, 10:1, 5:1, 1:1, 1:5 and 1:40, the SR range was from 0.54 to 1.28, which showed additive inhibition. When the mass ratio of the mixture of fludioxonil and tebuconazole was 1:10 and 1:20, the SR was 1.56 and 1.62, respectively, which showed synergistic inhibition. When the mass ratio of the mixture of fludioxonil and tebuconazole was 1:20, the SR value was the highest, which was the best synergistic ratio. These results could provide a theoretical basis for the control of wheat scab and delaying the development of tebuconazole resistance.

Key words: fludioxonil, tebuconazole, wheat scab, Fusarium graminearum, synergistic effect

中图分类号:

S482.2

姜佳, 陈金鹏, 魏江桥, 郭旭昊, 车志平, 田月娥, 陈根强, 刘圣明. 咯菌腈与戊唑醇复配对小麦赤霉病菌的增效作用研究[J]. 中国农学通报, 2022, 38(6): 116-120.

JIANG Jia, CHEN Jinpeng, WEI Jiangqiao, GUO Xuhao, CHE Zhiping, TIAN Yue’e, CHEN Genqiang, LIU Shengming. Synergistic Effect of Fludioxonil and Tebuconazole Against Fusarium graminearum[J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 116-120.

图/表 3

参考文献 15

[1]	张洪滨, 柳金伟, 刘秉江, 等. 山东省小麦赤霉病菌种群组成及其致病力分化[J]. 植物保护学报, 2013, 40(1):27-32.
[2]	程顺和, 张勇, 别同德, 等. 中国小麦赤霉病的危害及抗性遗传改良[J]. 江苏农业学报, 2012, 28(5):938-942.
[3]	陈怀谷, 蔡志祥, 陈飞, 等. 不同小麦品种抗赤霉病性类型和抗毒素积累能力分析[J]. 植物保护学报, 2007(1):32-36.
[4]	贾娇, 苏前富, 孟玲敏, 等. 抗咯菌腈禾谷镰刀菌的紫外诱导及其生物学特性[J]. 植物保护, 2016, 42(4):43-47,63.
[5]	邵莒南, 徐大同, 李慧, 等. 氟啶胺等杀菌剂对不同发育阶段小麦赤霉病菌的毒力及其作用方式[J]. 植物保护学报, 2016, 43(2):314-320.
[6]	纪军建, 王文桥, 张小风, 等. 连续施药及室内药剂驯化对番茄灰葡萄孢对咯菌腈敏感性的影响[J]. 农药学学报, 2012, 14(1):35-41.
[7]	EMERY K M, SCHERM H, SAVELLE A T. Assessment of interactions between components of fungicide mixtures against Monilinia fructicola[J]. Crop Protection, 2002, 21(1):41-47. doi: 10.1016/S0261-2194(01)00062-X URL
[8]	姚昕, 秦文. 苯醚甲环唑与异菌脲复配对石榴干腐病菌的联合毒力及贮藏期控制作用[J]. 果树学报, 2017, 34(8):1033-1042.
[9]	刘学敏, 李立军. 杀菌剂混剂的增效作用[J]. 农药科学与管理, 2002(5):12-15.
[10]	谭放军, 吴声海, 李细高, 等. 咯菌腈悬浮种衣剂在辣椒种子上的应用效果[J]. 辣椒杂志, 2020, 18(3):10-13.
[11]	徐建强, 杨岚, 平忠良, 等. 中国河南省小麦纹枯病菌对咯菌腈的敏感性[J]. 农药学学报, 2017, 19(5):563-568.
[12]	徐建强, 平忠良, 马世闯, 等. 河南省小麦赤霉病菌对咯菌腈的敏感性[J]. 植物保护学报, 2018, 45(6):1367-1373.
[13]	韩青梅, 康振生, 黄丽丽, 等. 戊唑醇对赤霉病菌生长发育影响的细胞学和免疫细胞化学研究[J]. 菌物学报, 2004(4):580-589.
[14]	徐建强, 张勇, 平忠良, 等. 河南省小麦赤霉病菌对戊唑醇的敏感性[J]. 麦类作物学报, 2018, 38(10):1255-1261.
[15]	宋阳阳, 林杨, 罗汉钢, 等. 湖北省小麦赤霉病菌对多菌灵、戊唑醇和咪鲜胺的敏感性[J]. 农药学学报, 2016, 18(6):724-728.

药剂名称	药剂比例	药剂浓度/(μg/mL)
咯菌腈	-	0,0.003125,0.00625,0.0125,0.025,0.05,0.1
戊唑醇	-	0,0.0625,0.125,0.25,0.5,1,2
咯菌腈: 戊唑醇	40:1	0,0.003125,0.00625,0.0125,0.025,0.05,0.1
咯菌腈: 戊唑醇	20:1	0,0.003125,0.00625,0.0125,0.025,0.05,0.1
咯菌腈: 戊唑醇	10:1	0,0.0078125,0.015625,0.03125,0.0625,0.0125
咯菌腈: 戊唑醇	5:1	0,0.0078125,0.015625,0.03125,0.0625,0.0125
咯菌腈: 戊唑醇	1:1	0,0.003125,0.00625,0.0125,0.025,0.05,0.1,0.2
咯菌腈: 戊唑醇	1:5	0,0.0625,0.0125,0.25,0.5,1
咯菌腈: 戊唑醇	1:10	0,0.0625,0.0125,0.25,0.5,1,2
咯菌腈: 戊唑醇	1:20	0,0.0625,0.0125,0.25,0.5,1,2
咯菌腈: 戊唑醇	1:40	0,0.0625,0.0125,0.25,0.5,1,2

药剂名称	药剂比例	药剂浓度/(μg/mL)
咯菌腈	-	0,0.003125,0.00625,0.0125,0.025,0.05,0.1
戊唑醇	-	0,0.0625,0.125,0.25,0.5,1,2
咯菌腈: 戊唑醇	40:1	0,0.003125,0.00625,0.0125,0.025,0.05,0.1
咯菌腈: 戊唑醇	20:1	0,0.003125,0.00625,0.0125,0.025,0.05,0.1
咯菌腈: 戊唑醇	10:1	0,0.0078125,0.015625,0.03125,0.0625,0.0125
咯菌腈: 戊唑醇	5:1	0,0.0078125,0.015625,0.03125,0.0625,0.0125
咯菌腈: 戊唑醇	1:1	0,0.003125,0.00625,0.0125,0.025,0.05,0.1,0.2
咯菌腈: 戊唑醇	1:5	0,0.0625,0.0125,0.25,0.5,1
咯菌腈: 戊唑醇	1:10	0,0.0625,0.0125,0.25,0.5,1,2
咯菌腈: 戊唑醇	1:20	0,0.0625,0.0125,0.25,0.5,1,2
咯菌腈: 戊唑醇	1:40	0,0.0625,0.0125,0.25,0.5,1,2

药剂名称	药剂比例	毒力回归方程	相关系数(r)	EC₅₀/(μg/mL)
咯菌腈	-	Y=7.7882+1.6519X	0.9904	0.0205
戊唑醇	-	Y=5.8324+1.0830X	0.9992	0.1704
咯菌腈: 戊唑醇	40:1	Y=6.9598+1.0972X	0.9412	0.0164
咯菌腈: 戊唑醇	20:1	Y=8.4929+2.1976X	0.9813	0.0257
咯菌腈: 戊唑醇	10:1	Y=7.0099+1.4526X	0.9917	0.0413
咯菌腈: 戊唑醇	5:1	Y=7.4310+1.5807X	0.9973	0.0290
咯菌腈: 戊唑醇	1:1	Y=6.7593+1.2498X	0.9870	0.0391
咯菌腈: 戊唑醇	1:5	Y=6.1071+1.1370X	0.9946	0.2805
咯菌腈: 戊唑醇	1:10	Y=5.9832+0.8316X	0.9940	0.0657
咯菌腈: 戊唑醇	1:20	Y=5.9918+0.8796X	0.9981	0.0782
咯菌腈: 戊唑醇	1:40	Y=5.9951+1.1876X	0.9917	0.1452

药剂名称	药剂比例	毒力回归方程	相关系数(r)	EC₅₀/(μg/mL)
咯菌腈	-	Y=7.7882+1.6519X	0.9904	0.0205
戊唑醇	-	Y=5.8324+1.0830X	0.9992	0.1704
咯菌腈: 戊唑醇	40:1	Y=6.9598+1.0972X	0.9412	0.0164
咯菌腈: 戊唑醇	20:1	Y=8.4929+2.1976X	0.9813	0.0257
咯菌腈: 戊唑醇	10:1	Y=7.0099+1.4526X	0.9917	0.0413
咯菌腈: 戊唑醇	5:1	Y=7.4310+1.5807X	0.9973	0.0290
咯菌腈: 戊唑醇	1:1	Y=6.7593+1.2498X	0.9870	0.0391
咯菌腈: 戊唑醇	1:5	Y=6.1071+1.1370X	0.9946	0.2805
咯菌腈: 戊唑醇	1:10	Y=5.9832+0.8316X	0.9940	0.0657
咯菌腈: 戊唑醇	1:20	Y=5.9918+0.8796X	0.9981	0.0782
咯菌腈: 戊唑醇	1:40	Y=5.9951+1.1876X	0.9917	0.1452

咯菌腈:戊唑醇	EC₅₀(Obs)/(μg/mL)	EC₅₀(Exp)/(μg/mL)	增效系数(SR)
40:1	0.0164	0.0209	1.28
20:1	0.0257	0.0214	0.83
10:1	0.0413	0.0223	0.54
5:1	0.0290	0.0240	0.83
1:1	0.0391	0.0366	0.94
1:5	0.1063	0.0768	0.72
1:10	0.0657	0.1024	1.56
1:20	0.0782	0.1264	1.62
1:40	0.1452	0.1446	1.00

咯菌腈与戊唑醇复配对小麦赤霉病菌的增效作用研究

Synergistic Effect of Fludioxonil and Tebuconazole Against Fusarium graminearum

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