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中国农学通报 ›› 2022, Vol. 38 ›› Issue (9): 14-19.doi: 10.11924/j.issn.1000-6850.casb2021-0871

所属专题: 生物技术 小麦

• 农学·农业基础科学 • 上一篇    下一篇

外源活性物质对小麦苗期抗逆性的影响

武迪1(), 张锋1,2, 隋春莹1, 师君慧1, 万雪洁1, 刘义国1, 韩伟3, 师长海1()   

  1. 1青岛农业大学农学院,山东省旱作农业技术重点实验室,山东青岛 266109
    2中国科学院东北地理与农业生态研究所,长春 130102
    3山东省农业技术推广中心,济南 250100
  • 收稿日期:2021-09-10 修回日期:2021-11-03 出版日期:2022-03-25 发布日期:2022-04-02
  • 通讯作者: 师长海
  • 作者简介:武迪,女,1996年出生,河北冀州人,硕士研究生,主要从事作物抗旱耐盐机理研究。通信地址:266109 青岛市城阳区长城路700号 青岛农业大学农学院,Tel:0532-58957447,E-mail: 18730899456@163.com
  • 基金资助:
    山东省重点研发计划“冬小麦滴灌分层施肥及水溶肥有机替代技术研究与示范”(2017CXGC0307);山东省高等学校青创科技支持计划“小麦抗旱耐盐品种选育及配套栽培技术研发”(2019KJF013)

Exogenous Active Substances: Effect on Stress Resistance of Wheat Seedling

WU Di1(), ZHANG Feng1,2, SUI Chunying1, SHI Junhui1, WAN Xuejie1, LIU Yiguo1, HAN Wei3, SHI Changhai1()   

  1. 1Qingdao Agricultural University, Shandong Province Key Laboratory of Dry Farming Technology, Qingdao, Shandong 266109
    2Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102
    3Agricultural Technology Extension Center of Shandong Province, Jinan 250100
  • Received:2021-09-10 Revised:2021-11-03 Online:2022-03-25 Published:2022-04-02
  • Contact: SHI Changhai

摘要:

为了探讨外源活性物质对小麦耐盐性的影响,采用盐碱土盆栽法,分析了大丽轮枝孢(Verticillium dahliae) Asp f2类蛋白溶液(0.8%,VDAL)、海藻寡糖(0.1%,AOS)和蒸馏水(CK)拌种对小麦幼苗生长发育和抗逆生理的影响。结果表明,与CK相比,VDAL和AOS拌种均显著提高了盐胁迫下小麦苗期株高、根系干重和叶干重,根冠比分别较CK提高8.5%和6.8%。AOS拌种显著提高了叶片叶绿素a、叶绿素a+b和类胡萝卜素含量。VDAL和AOS拌种均能显著降低叶片丙二醛含量,VDAL拌种能显著提高叶片可溶性糖和脯氨酸含量,使叶片过氧化物酶(POD)活性显著提高达29.5%;AOS拌种能显著提高脯氨酸含量和POD活性。由此可见,VDAL和AOS拌种均对提高小麦苗期耐盐性具有一定促进作用,可以考虑在盐碱地小麦栽培中应用,以提高群体量。

关键词: 小麦, 外源活性物质, 盐碱胁迫, 海藻寡糖, 耐盐性

Abstract:

The aim is to investigate the effect of exogenous active substances on salt tolerance of wheat. The effects of Asp f2 protein solution of Verticillium dahliae (0.8%, VDAL), algal oligosaccharides (0.1%, AOS) and distilled water (CK) seed dressing on the growth and stress-resistance physiology of wheat seedlings were analyzed with saline-alkali soil pot experiment. The results showed that compared with CK, VDAL and AOS seed dressing significantly increased plant height, root dry weight and leaf dry weight at seedling stage, and the root shoot ratio increased by 8.5% and 6.8%, respectively. AOS seed dressing significantly increased the contents of chlorophyll a, chlorophyll a+b and carotenoid in leaves. VDAL and AOS seed dressing could significantly reduce malondialdehyde content in leaves. VDAL seed dressing could significantly increase soluble sugar and proline content in leaves, and increase peroxidase (POD) activity in leaves by 29.5%. AOS seed dressing could significantly increase proline content and POD activity. In conclusion, both VDAL and AOS seed dressing could promote the salt tolerance of wheat seedling, and be applied to wheat cultivation in saline-alkali land to increase the population size of wheat.

Key words: wheat, exogenous active substances, saline-alkali stress, algal oligosaccharides, salt tolerance

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