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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (18): 205-208.doi: 10.11924/j.issn.1000-6850.2012-0555

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

高活性纤维素酶的分离纯化

李志平 杨盛 侯红萍   

  • 收稿日期:2012-02-23 修回日期:2012-04-09 出版日期:2012-06-25 发布日期:2012-06-25
  • 基金资助:

    山西省科技厅攻关项目

Purification of Highly Active Cellulase

  • Received:2012-02-23 Revised:2012-04-09 Online:2012-06-25 Published:2012-06-25

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摘要：

为了纯化纤维素酶粗酶液，通过采用硫酸铵分级沉淀、Sephadex G-100凝胶柱层析、DEAF FF弱阴离子交换柱层析的方法，研究纤维素酶粗酶液分离纯化的方法。结果表明：采用硫酸铵分级沉淀、Sephadex G-100凝胶柱层析、DEAF FF弱阴离子交换柱层析的方法对内切葡聚糖苷酶组分的分离纯化有效。通过试验研究得到一种内切葡聚糖苷酶组分，其比活力提高了5.89倍，回收率为22%。

关键词: 污染评价, 污染评价

Abstract:

To study the separation and purification of cellulase from crude enzyme, the method of ammonium sulfate fractionation, Sephadex G-100 gel filtration and DEAF FF weak anion exchange column chromatography method were used. The results showed that: the usage of ammonium sulfate fractionation, Sephadex G-100 gel column chromatography, DEAF FF weak anion exchange column chromatography is a efficient method to purify CMCase. The specific activity of CMCase increase 5.89 times purifed in this way, while the recovery rate was 22%.

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