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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (21): 272-276.doi: 10.11924/j.issn.1000-6850.2012-0797

所属专题： 小麦

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不同碱性提取剂对小麦麸皮水不溶性阿拉伯木聚糖提取效果的比较研究

李雪 苏东民 苏东海 赵仁勇 魏雪芹 马四平 邢丽艳   

  • 收稿日期:2012-03-08 修回日期:2012-04-26 出版日期:2012-07-25 发布日期:2012-07-25
  • 基金资助:

    国家自然科学基金项目;北京市属高等学校人才强教计划资助项目;河南工业大学2010年研究生科技创新基金项目

Investigation on the extraction of Water-unextractable arabinoxylans from wheat bran using different alkaline extractants

  • Received:2012-03-08 Revised:2012-04-26 Online:2012-07-25 Published:2012-07-25

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摘要：

为了比较不同碱性提取剂对小麦麸皮水不溶性阿拉伯木聚糖(B-WUAX)产率以及终产物组成的影响，试验选取5种碱性提取剂，结合各学者优化的最佳提取方法对B-WUAX进行提取。并对提取产物总糖、蛋白质、阿拉伯木聚糖以及阿魏酸含量进行了测定。结果表明：以饱和氢氧化钡作为提取剂时，其粗B-WUAX产率以及B-WUAX含量均为最高值，其值分别为14.3%、78.90%。饱和氢氧化钡对B-WUAX的选择性最高，其适合于实验室对B-WUAX的研究；B-WUAX产率与提取溶剂的pH值无关；产物中阿魏酸含量与提取溶剂的碱性强度有关，碱性越强产物阿魏酸含量越低。当以碱性电解水和含双氧水的氢氧化钠溶液为提取溶剂时，提取物阿魏酸含量分别为1220 μg/g和1064 μg/g，而以饱和氢氧化钡作为溶剂对B-WUAX进行提取时，产物中阿魏酸的含量仅为53.3 μg/g。氢氧化钠由于工艺简单，成本低，产率适当，可能更适合B-WUAX提取的工业化生产。

关键词: 产量与品质, 产量与品质

Abstract:

In order to compare the influence of different alkaline extractions on the yield of water-unextractable arabinoxylans from wheat bran (B-WUAX), and on the composition of alkaline extract, five different alkaline extractions were used to extract B-WUAX. Combining with the optimum extraction method for B-WUAX, the content of total sugar, protein, B-WUAX and ferulic acid in the alkaline extract were determined. The results showed that using saturated barium hydroxide as the extraction the highest yield and content of B-WUAX were obtained, and the values were 14.3%, 78.90% respectively. Saturated barium hydroxide had the highest selectivity of B-WUAX which was more suitable as the extraction to study B-WUAX in laboratory. There was no relationship between the yield of B-WUAX and the pH of the extraction but a high relationship between the ferulic acid content and alkalinity of extraction. Extracting B-WUAX with stronger alkaline extraction, lower ferulic acid content was obtained. Using alkaline electrolysis water and sodium hydroxide solution of hydrogen peroxide as extraction, the ferulic acid content were 1220 μg/g and 1064 μg/g respectively. Using saturated barium hydroxide as the extraction, the ferulic acid content was 53.3 μg/g. Due to more simple extraction process, lower cost, and proper yield, sodium hydroxide was more suitable as the extraction to produce B-WUAX at large scale.

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