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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (12): 80-90.doi: 10.11924/j.issn.1000-6850.2012-1663

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

甘蔗葡聚糖酶ScBG1基因的电子克隆及序列分析

薛扳佟 郭晋隆 苏亚春 罗俊 游倩 阙友雄   

  • 收稿日期:2012-05-02 修回日期:2012-06-23 出版日期:2013-04-25 发布日期:2013-04-25

In silico cloning and sequence analysis of glucanase gene（ScBG1） from sugarcane

  • Received:2012-05-02 Revised:2012-06-23 Online:2013-04-25 Published:2013-04-25

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摘要： 为了揭示甘蔗β-1,3-葡聚糖酶基因的结构特点和功能特性，从而为该基因的研究和应用提供基础。本研究以水稻β-1,3-葡聚糖酶基因(U72255.1)为搜寻探针，运用电子克隆技术，获得一条甘蔗β-1,3-葡聚糖酶基因全长cDNA，命名为ScBG1，并对其进行生物信息学分析。结果表明：该基因的cDNA序列全长为1842 bp，包含一个1488 bp完整的开放读码框，编码495个氨基酸残基，属于糖基水解酶第十七家族，为疏水性蛋白，含有信号肽。将ScBG1与其他植物种的β-1,3-葡聚糖酶基因进行序列比对，发现该基因具有保守和典型的葡聚糖酶结构域，碱基序列的保守性为45%~73%。研究结果为该基因的实验克隆奠定基础，也为甘蔗β-1,3-葡聚糖酶基因的分子功能研究提供参考。

关键词: 分子印迹, 分子印迹

Abstract: In order to reveal the structure and function characteristics of β-1,3-glucanase gene in sugarcane, and provide the basis for the research and application of this gene, a sugarcane full-length cDNA sequence of β-1,3-glucanase gene, termed as ScBG1, was obtained in silico by using the rice β-1,3- glucanase gene (U72255.1) as search probe, and the bioinformatics analysis was also finished. The results showed that the full-length of the cDNA sequence of the ScBG1 gene was 1842 bp, which contained a 1488 bp complete open reading frame and enco ded 495 amino acid residues. The assemble gene belonged to the 17th glycol hydro family and hydrophobic protein containing the signal peptide. With several plants’ β-1,3-glucanase Based on the sequences comparison of the ScBG1 gene with β-1,3-glucanase genes from several other plant species, the ScBG1 gene had the typical structural region of β-1,3-glucanase and was high conserved with 45%-73% sequence similarity. The results in this paper provided the basis for experimental isolation of the ScBG1 gene in sugarcane and the reference for the research of its molecule function.