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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (36): 310-314.doi: 10.11924/j.issn.1000-6850.2014-2359

所属专题： 玉米

• 食品 营养 检测 安全 • 上一篇    下一篇

分子印迹技术在线富集测定玉米中嗪草酮残留

郑振山,张少军,温之雨,陈勇达,王莉,钱训   

  1. 河北省农林科学院遗传生理研究所,河北省农林科学院遗传生理研究所,河北省农林科学院遗传生理研究所,河北省农林科学院遗传生理研究所,河北省农林科学院遗传生理研究所,河北省农林科学院遗传生理研究所
  • 收稿日期:2014-08-29 修回日期:2014-08-29 接受日期:2014-10-14 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 张少军
  • 基金资助:
    河北省农林科学院青年基金“分子印迹技术在三嗪类除草剂残留分析中的应用研究”(A2012110202)。

Determination of Metribuzin Residues in Corn with On-line Preconcentration by Molecular Imprinting Technique

  • Received:2014-08-29 Revised:2014-08-29 Accepted:2014-10-14 Online:2015-03-20 Published:2015-03-20

摘要： 为了对玉米中的嗪草酮残留进行高效的分离、富集和测定，以嗪草酮为模板分子，采用原位聚合法制备了分子印迹聚合物，并将印迹聚合物作为固相萃取柱用于玉米样品的前处理过程，建立了在线富集液相色谱法测定玉米中嗪草酮残留的分析方法。研究结果表明，嗪草酮印迹聚合物具有良好的亲和性和特异选择性，在0.01~0.50 μg/mL质量浓度范围内有良好的线性关系(r=9991)，最低检出浓度(S/N=3)为1.8×10-3 μg/mL，在0.01，0.05，0.10 mg/kg 3个浓度的添加水平下，方法的平均回收率在80.9%~94.5%之间，相对标准偏差低于5.98%。方法简化了样品前处理步骤，提高了检测灵敏度度，分析时间短，无基质干扰，精密度和准确度高，可应用于玉米中嗪草酮残留的分析检测。

关键词: 测土配方施肥技术, 测土配方施肥技术, 低碳农业, 科学施肥, 农业可持续发展, 农技推广

Abstract: For better resolution, enrichment and determinaiton of metribuzin residues in corn, a molecularly imprinted polymer with metribuzin as a template molecule synthesized by in situ polymerization was used as a solid-phase extraction sorbent for the on-line preconcentration and analysis of metribuzin residues in corn by liquid chromatography. The results showed that the molecularly imprinted polymer had significant molecularly imprinting effect and had high affinity and selectivity for metribuzin in water-containing system. Good linearity was obtained for metribuzin at the concentration of 0.01-0.50μg/mL with correlation coefficient of 0.9991. The detection limit (S/N=3) of the method for metribuzin was 1.8×10- 3μg/mL, the recoveries of metribuzin in corn at 0.01, 0.05 and 0.10 mg/kg were 80.9%- 94.5% and the relative standard deviations were less than 5.98%. The pretreatment procedure was simplified and the sensitivity was enhanced by using the method. The method was sensitive, reproducible, reliable, timesaving and no matrix interference. It could be used on the determination of metribuzin residues in corn.