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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (29): 83-86.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪OGP蛋白基因克隆及分泌载体的构建与鉴定

李方正   

  • 收稿日期:2012-05-08 修回日期:2012-06-21 出版日期:2012-10-15 发布日期:2012-10-15
  • 基金资助:

    雌二醇对输卵管上皮细胞钙网蛋白表达的影响及调控的信号途径

Clone and Construction of Secretory Expression Vector of Porcine OGP

  • Received:2012-05-08 Revised:2012-06-21 Online:2012-10-15 Published:2012-10-15

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摘要：

为了获得重组猪OGP蛋白，构建OGP分泌表达载体，以使猪输卵管特异性糖蛋白在体外受精中发挥重要作用。从猪输卵管中提取总RNA，运用RT-PCR技术扩增猪OGP全部编码序列，克隆到pET22b载体中，构建成pET22b-OGP分泌表达载体，对其进行酶切、PCR和测序鉴定。结果表明：成功构建了OGP分泌表达载体，为进一步研究该OGP在猪体外受精中的功能奠定了基础。

关键词: 存活率, 存活率

Abstract:

The OGP protein was important in the in vitro fertilization of pig. In order to produce the recombination OGP protein, the OGP secretory expression vector was constructed. The total RNA was extracted from porcine oviduct and coding sequence of porcine OGP was amplified using RT-PCR method. The OGP CDS was cloned into the pET22b vector and then identified by restriction enzyme analysis, PCR amplification and DNA sequencing. The results showed that the pET22b-OGP vector was constructed successfully, which provided advantage for further study of OGP function at the porcine in vitro fertilization.