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中国农学通报 ›› 2014, Vol. 30 ›› Issue (16): 139-142.doi: 10.11924/j.issn.1000-6850.2014-0142

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

卷丹不同继代试管苗茎尖超低温保存效果及组织细胞学研究

沈琛 徐瑾 李秉玲 刘燕   

  • 收稿日期:2014-01-14 修回日期:2014-02-21 出版日期:2014-06-05 发布日期:2014-06-05
  • 基金资助:
    国家自然基金项目 “花粉超低温保存差异蛋白在氧化应激和细胞凋亡中作用研究” (31370693)。

Effect of Successive Subculture Shoot Tips in Vitro of Lilium lancifolium on Cryopreservation

  • Received:2014-01-14 Revised:2014-02-21 Online:2014-06-05 Published:2014-06-05

摘要: 为了研究组培茎尖的继代次数对其超低温保存后存活的影响,以卷丹试管苗不同继代次数的茎尖为材料,采用玻璃化法进行了超低温保存效果比较研究。结果表明,1~4代试管苗茎尖冻后存活率分别为55.8%、93.3%、67.0%和64.1%。同时,即初代培养后继代培养2个月的第2代试管苗茎尖超低温保存后存活率显著高于其他继代培养子组培苗。对各代试管苗茎尖进行石蜡连续纵切片显微观察,结果表明2代试管苗顶端分生组织细胞形态不同于其他继代代数,其显著特征是细胞体积小、细胞核大且细胞质浓密,这可能是其茎尖超低温保存显著高于其他3代的原因。

关键词: 磺胺类药物, 磺胺类药物, 胶体金, 快速检测, 水产品

Abstract: In order to study the effect of subculture times of shoot tips on cryopreservation, the survival rates of 1-4 succesive subculture shoot tips in vitro of Lilium lancifolium after cryopreservation were compared. As the results showed, the survival rates were 55.8%, 93.3%, 67.0% and 64.1%. What’s more, the second generation subcultures which had been subcultured two months got the higher survival rate than other generations. However, continuous paraffin sections of shoot tips of every generation were investigated by microscopy, the results showed the second generation plantlets was found different from others in morphological studies of protomerism cell, which involved smaller cell volume, larger cell nuclear and richer cytoplasm, and this may lead to the higher survival rate of the second generation after cryopreservation than others .