免疫分析技术在农药残留快速检测中的应用及研究进展

doi:10.11924/j.issn.1000-6850.casb2020-0419

中国农学通报 ›› 2021, Vol. 37 ›› Issue (23): 106-112.doi: 10.11924/j.issn.1000-6850.casb2020-0419

所属专题：植物保护

• 食品·营养·检测·安全 • 上一篇下一篇

免疫分析技术在农药残留快速检测中的应用及研究进展

郭思依(), 孙明娜, 董旭, 褚玥, 童舟, 王梅, 高同春, 段劲生()

安徽省农业科学院植物保护与农产品质量安全研究所/农业农村部农产品质量安全风险评估实验室（合肥）,合肥 230031

收稿日期:2020-09-02 修回日期:2020-12-23 出版日期:2021-08-15 发布日期:2021-08-26
通讯作者: 段劲生
作者简介:郭思依,女,1992年出生,硕士,研究实习员,研究方向为农药残留及农产品质量安全。通信地址:230031 安徽省农业科学院, E-mail: guosiyi1101@126.com。
基金资助:
安徽省农科院团队项目“农产品质量安全检测技术与风险评估”(2020YL076);安徽省农业生态环保与质量安全产业技术体系(AHCYJSTX-15);国家重点研发计划“大豆种植体系中农药沉降行为及减施因素”(2016YFD0200207-4)

Application and Research Progress of Immunoassay Technology in Rapid Detection of Pesticide Residues

Guo Siyi(), Sun Mingna, Dong Xu, Chu Yue, Tong Zhou, Wang Mei, Gao Tongchun, Duan Jinsheng()

Institute of Plant Protection and Agricultural Product Quality and Safety, Anhui Academy of Agricultural Sciences/Key Laboratory of Agro-Product Safety Risk Evaluation (Hefei), Hefei 230031

Received:2020-09-02 Revised:2020-12-23 Online:2021-08-15 Published:2021-08-26
Contact: Duan Jinsheng

摘要/Abstract

摘要：

为解决色谱法等常规方法在农药残留检测中耗时长、成本高等问题,提出了基于免疫分析的简便快捷、高选择性和高灵敏度的农药残留快检分析方法。综述了常用的农药残留快速检测技术,包括酶联免疫吸附测定、荧光免疫分析、免疫层析、免疫磁珠、仿生免疫分析及生物条形码技术等的作用原理、特点及研究进展。不同的方法有着各自的优缺点和适用范围,而分子印迹、纳米技术和基因检测技术的快速发展,为农药残留免疫分析提供了较高的灵敏度和准确度。建议基于免疫分析技术的自身优势,结合新技术并不断完善,进而在农药残留快检领域发挥重要作用。未来在农产品质量安全检测和控制上,开发简单便携的小型化和集成化检测手段,提高方法和仪器的稳定性和灵敏度成为农药残留快速检测的发展趋势。

关键词: 农产品, 免疫分析, 农药残留, 快速检测, 研究进展

Abstract:

In order to solve the problems of time-consuming and high cost in the detection of pesticide residues by conventional methods such as chromatography, a simple, fast, high selectivity and high sensitivity rapid detection method for pesticide residues based on immunoassay was proposed. This review summarized the principles, characteristics and research progress of commonly used rapid detection techniques for pesticide residues, including enzyme-linked immunosorbent assay, fluorescence immunoassay, immunochromatography, immunomagnetic beads, biomimetic immunoassay and bio-barcode assay. Different methods have their own advantages, disadvantages and scope of application, and the rapid development of molecular imprinting, nanotechnology and genetic detection technology could provide high sensitivity and accuracy for pesticide residue immunoassay. Based on the advantages of immunoassay technology, it will play an important role in the field of rapid pesticide residue detection combined with new technology and continuous improvement. The miniaturization and integration of the detection methods, and higher stability and sensitivity of detect instruments are the development direction of pesticide residue detection in the future.

Key words: agricultural products, immunoassay, pesticide residues, rapid detection, review

中图分类号:

S481.8

郭思依, 孙明娜, 董旭, 褚玥, 童舟, 王梅, 高同春, 段劲生. 免疫分析技术在农药残留快速检测中的应用及研究进展[J]. 中国农学通报, 2021, 37(23): 106-112.

Guo Siyi, Sun Mingna, Dong Xu, Chu Yue, Tong Zhou, Wang Mei, Gao Tongchun, Duan Jinsheng. Application and Research Progress of Immunoassay Technology in Rapid Detection of Pesticide Residues[J]. Chinese Agricultural Science Bulletin, 2021, 37(23): 106-112.

图/表 2

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免疫分析技术在农药残留快速检测中的应用及研究进展

Application and Research Progress of Immunoassay Technology in Rapid Detection of Pesticide Residues

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