杀线虫剂新品种及作用机制研究进展

doi:10.11924/j.issn.1000-6850.casb2021-1045

摘要/Abstract

摘要：

植物寄生线虫每年在世界范围内造成巨大损失,在众多防治措施中,化学防治依然是防治线虫的主要手段。本研究按照杀虫剂和杀菌剂抗性行动委员会（IRAC和FRAC）对杀线虫剂作用机制的分类,对部分传统与新杀线虫剂的作用机制研究进行了总结,以期能够为杀线虫剂产品的研究提供理论基础。

关键词: 植物寄生线虫, 杀线虫剂, 作用机制, 农药登记, 研究进展

Abstract:

Plant-parasitic nematodes cause huge losses worldwide every year, and among the many prevention and control measures, chemical control is still the main one. According to the action mechanism of nematicides classified by the Insecticide and Fungicide Resistance Action Committee (IRAC and FRAC), this study summarized the action mechanisms of some traditional and new nematicides, in order to provide a theoretical basis for the research of nematicides.

Key words: plant-parasitic nematode, nematicide, mechanism of action, pesticide registration, research progress

中图分类号:

S482.99

王晴, 方文生, 李园, 王秋霞, 颜冬冬, 曹坳程. 杀线虫剂新品种及作用机制研究进展[J]. 中国农学通报, 2022, 38(30): 100-107.

WANG Qing, FANG Wensheng, LI Yuan, WANG Qiuxia, YAN Dongdong, CAO Aocheng. Advances in New Nematicides and Their Action Mechanism[J]. Chinese Agricultural Science Bulletin, 2022, 38(30): 100-107.

图/表 2

参考文献 24

[25]	YATES D M, PORTILLO V, WOLSTENHOLME A J. The avermectin receptors of Haemonchus contortus and Caenorhabditis elegans[J]. International journal for parasitology, 2003, 33(11):1183-1193. doi: 10.1016/S0020-7519(03)00172-3 URL
[26]	CULLY D, VASSILATIS D, LIU K, et al. Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans[J]. Nature, 1994, 371:707-711. doi: 10.1038/371707a0 URL
[27]	WOLSTENHOLME A J, ROGERS A T. Glutamate-gated chloride channels and the mode of action of the avermectin/milbemycin anthelmintics[J]. Parasitology, 2005, 131 Suppl:S85-S95. doi: 10.1017/S0031182005008218 pmid: 16569295
[28]	LAUGHTON DL, LUNT GG, WOLSTENHOLME AJ. Reporter gene constructs suggest that the Caenorhabditis elegans avermectin receptor beta-subunit is expressed solely in the pharynx[J]. J exp biol, 1997, 200(Pt 10):1509-14. doi: 10.1242/jeb.200.10.1509 URL
[29]	VELOUKAS T, KARAOGLANIDIS G S. Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity[J]. Pest management science, 2012, 68(6):858-864. doi: 10.1002/ps.3241 URL
[30]	HEIKEN J A. The efects of fuopyram on nematodes[D]. Raleigh: North Carolina State University, 2017:41-44.
[31]	周大纲. 新一代优秀杀线虫剂氟吡菌酰胺[J]. 世界农药, 2016, 38(5):61-63.
[32]	OKA Y, SAROYA Y. Effect of fluensulfone and fluopyram on the mobility and infection of second-stage juveniles of Meloidogyne incognita and M. javanica[J]. Pest manag sci, 2019, 75(8):2095-2106.
[33]	FASKE T R, HURD K. Sensitivity of Meloidogyne incognita and Rotylenchulus reniformis to fluopyram[J]. J nematol, 2015, 47(4):316-321.
[34]	MCLEOD R W, KHAIR G T. Effects of oximecarbamate, organophosphate and benzimidazole nematicides on life cycle stages of root-knot nematodes, Meloidogyne spp.[J]. Annals of applied biology, 1975, 79(3):329-341. doi: 10.1111/j.1744-7348.1975.tb01589.x URL
[35]	YU L, WEIPING Z, YING W, et al. Oxidative stress, intestinal damage, and cell apoptosis: toxicity induced by fluopyram in Caenorhabditis elegans[J]. Chemosphere, 2022, 3(286):131830.
[36]	RANA S. Syngenta presents tymirium nematicide/fungicide technology[J]. AGROW world crop protection news, 2020:4.
[37]	党铭铭, 郭永艳, 田雁飞, 等. 2020年最新公开的含氟杀虫剂[J]. 农药, 2021, 60(3):157-161.
[38]	UMETSU N, SHIRAI Y. Development of novel pesticides in the 21st century[J]. Journal of pesticide science, 2020, 45(2).
[39]	叶萱. 具新颖作用机制杀虫杀螨剂——螺虫乙酯[J]. 世界农药, 2011, 33(5):54-55.
[40]	BAYER CROP SCIENCE. Movento MPC insecticide key pests[EB/OL]. https://www.cropscience.bayer.us/products/insecticides/movento-mpc/pest. 2021-06-16/2021-11-04.
[41]	OPPERMAN C H, VANG L E, SCHWARZ M R, et al. Spirotetramat causes an arrest of nematode juvenile development[J]. Nematology, 2015:1-11.
[42]	GUTBROD P, GUTBROD K, NAUEN R, et al. Inhibition of acetyl-CoA carboxylase by spirotetramat causes growth arrest and lipid depletion in nematodes[J]. Scientific reports, 2020:12710.
[43]	PHILLION D P, YALAMANCHILI G, RUMINSKI P G, et al. Fluoroalkenyl compounds and their use as pest control agents[P]. AU3961195A,19960523.
[44]	KEARN J, LUDLOW E, DILLON J, et al. Fluensulfone is a nematicide with a mode of action distinct from anticholinesterases and macrocyclic lactones[J]. Pesticide biochemistry & physiology, 2014, 109(1):44-57.
[1]	BLAXTER M L. Nematoda: genes, genomes and the evolution of parasitism[J]. Adv parasitol, 2003, 54:101-195.
[2]	佩里. 植物线虫学[M].简恒,译. 北京: 中国农业大学出版社, 2011.
[45]	JAMES, KEARN, CATHERINE, et al. Progressive metabolic impairment underlies the novel nematicidal action of fluensulfone on the potato cyst nematode Globodera pallida[J]. Pesticide biochemistry & physiology, 2017.
[46]	ALKESH H, DIVYA S, VEERA V S K K, et al. Effect of fluensulfone on different functional genes of root-knot nematode Meloidogyne incognita[J]. Journal of nematology, 2021,53.
[47]	陈晨. 杀线虫剂Tioxazafen应用研究与开发进展[J]. 现代农药, 2018, 17(1):46-49.
[48]	顾林玲, 柏亚罗. 极具发展潜力的十大农药新品种的应用与开发[J]. 现代农药, 2018, 17(2):1-7.
[49]	LAHM G P, DESAEGER J, SMITH B K, et al. The discovery of fluazaindolizine: a new product for the control of plant parasitic nematodes[J]. Bioorganic & medicinal chemistry letters, 2017.
[50]	CHEN X, LI X, PANG K, et al. Dissipation behavior and residue distribution of fluazaindolizine and its seven metabolites in tomato ecosystem based on SAX SPE procedure using HPLC-QqQ-MS/MS technique[J]. Journal ofhazardous materials, 2018:342.
[51]	林宏芳. 新农药三氟咪啶酰胺在环境中的转化机理及其转化产物的生物毒性研究[D]. 北京: 北京科技大学, 2019.
[52]	陈品三. 杀线虫剂主要类型、特性及其作用机制[J]. 农药科学与管理, 2001(2):33-35.
[53]	ZHANG C, WU H, ZHAO Y, et al. Comparative studies on mitochondrial electron transport chain complexes of Sitophilus zeamais treated with allyl isothiocyanate and calcium phosphide[J]. Pestic biochem phys, 2016, 126:70-75. doi: 10.1016/j.pestbp.2015.07.009 URL
[54]	ZHANG C, MA Z Q, ZHANG X, et al. Transcriptomic alterations in Sitophilus zeamais in response to allyl isothiocyanate fumigation[J]. Pestic biochem phys, 2017, 137:62-70. doi: 10.1016/j.pestbp.2016.10.001 URL
[55]	YANG S, YJ A, HUA W B, et al. Function of four mitochondrial genes in fumigation lethal mechanisms of Allyl Isothiocyanate against Sitophilus zeamais adults[J]. Pesticide biochemistry and physiology, 2021, 179:104947. doi: 10.1016/j.pestbp.2021.104947 URL
[56]	曹坳程, 褚世海, 郭美霞, 等. 硫酰氟——溴甲烷土壤消毒潜在的替代品[J]. 农药学学报, 2002(3):91-93.
[3]	ABAD P, GOUZY J, AURY J M, et al. Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita[J]. Nat biotechnol, 2008, 26(8):909-915. doi: 10.1038/nbt.1482 URL
[4]	彭德良. 植物线虫病害:我国粮食安全面临的重大挑战[J]. 生物技术通报, 2021, 37(7):1-2.
[57]	姚玉荣, 霍建飞, 贲海燕, 等. 硫酰氟熏蒸剂对保护地西瓜根结线虫的防治效果评价[J]. 安徽农业科学, 2021, 49(6):143-144.
[58]	王佩圣, 程星, 张涛, 等. 硫酰氟熏蒸对保护地黄瓜根结线虫的防治效果[J]. 山东农业科学, 2014, 46(1):92-93.
[59]	MEIKLE R W, STEWART D, GLOBUS O A. Fumigant mode of action, drywood termite metabolism of vikane fumigant as shown by labeled pool technique[J]. J.agric.food chem, 1963, 11(3):226-230.
[60]	王秋霞, 颜冬冬, 王献礼, 等. 土壤熏蒸剂研究进展[J]. 植物保护学报, 2017, 44(4):529-543.
[61]	DONGDONG Y, AOCHENG C, QIUXIA W, et al. Dimethyl disulfide (DMDS) as an effective soil fumigant against nematodes in China[J]. Plos one, 2019, 14(10).
[62]	张大琪, 颜冬冬, 李青杰, 等. 二甲基二硫的生物活性评价及对土壤养分的影响[J]. 植物保护, 2020, 46(1):151-156.
[5]	SEONG J, SHIN J, KIM K, et al. Microbial production of nematicidal agents for controlling plant-parasitic nematodes[J]. Process biochemistry, 2021, 108(9):69-79. doi: 10.1016/j.procbio.2021.06.006 URL
[6]	BARGMANN C I. Neurobiology of the Caenorhabditis elegans Genome[J]. Science, 1998, 282:802-805.
[63]	SÉBASTIEN D, FRAN OISE G, DAVID M, et al. Dimethyl disulfide exerts insecticidal neurotoxicity through mitochondrial dysfunction and activation of insect K(ATP) channels.[J]. Journal of Neurophysiology, 2003, 90(1):259-270. pmid: 12843310
[64]	刘维志. 植物病原线虫学[M]. 北京: 中国农业出版社, 2000.
[7]	LEUNG M C, WILLIAMS P L, BENEDETTO A, et al. Caenorhabditis elegans: an emerging model in biomedical and environmental toxicology[J]. Toxicological sciences, 2008, 106(1):5-28. doi: 10.1093/toxsci/kfn121 pmid: 18566021
[8]	吴青松, 彭德良, 黄文坤. 植物寄生线虫乙酰胆碱酯酶结构与功能研究进展[J]. 植物保护, 2016, 42(3):17-21.
[65]	OKA Y. From Old-Generation to Next-Generation Nematicides[J]. Agronomy, 2020, 10(9):1387. doi: 10.3390/agronomy10091387 URL
[66]	SHIVAKUMARA T N, SOMVANSHI V S, PHANI V, et al. Meloidogyne incognita (Nematoda: Meloidogynidae) sterol-binding protein Mi-SBP-1 as a target for its management[J]. International journal for parasitology, 2019, 49(13-14):1061-1073. doi: 10.1016/j.ijpara.2019.09.002 URL
[9]	PIOTTE, ARTHAUD, ABAD, et al. Molecular cloning of an acetylcholinesterase gene from the plant parasitic nematodes, Meloidogyne incognita and Meloidogyne javanica[J]. MOL biochem parasitol, 1999, 99(2):247-256. doi: 10.1016/S0166-6851(99)00033-X URL
[10]	LAFFAIRE J B, JAUBERT S, ABAD P, et al. Molecular cloning and life stage expression pattern of a new acetylcholinesterase gene from the plant-parasitic nematode Meloidogyne incognita[J]. Nematology, 2003, 5(2):213-217. doi: 10.1163/156854103767139707 URL
[11]	丁中, 彭德良, 高必达, 等. 甘薯茎线虫乙酰胆碱酯酶基因Dd-ace-1全长cDNA的克隆和序列分析[J]. 湖南农业大学学报:自然科学版, 2010, 36(4):437-441.
[12]	丁中, 彭德良, 高必达, 等. 甘薯茎线虫乙酰胆碱酯酶基因ace-3全长cDNA的克隆和序列分析[J]. 农业生物技术学报, 2008(2):326-331.
[67]	沈德隆, 龚启孙, 翁建全, 等. 具有杀线虫活性的三氟丁烯类化合物研究进展[J]. 浙江化工, 2005(2):24-26.
[13]	丁中, 彭德良, 黄文坤, 等. 甘薯茎线虫乙酰胆碱酯酶基因Dd-ace-2全长cDNA的克隆和序列分析[J]. 生物工程学报, 2008(2):239-244.
[14]	COSTA J C, LILLEY C J, ATKINSON H J, et al. Functional characterisation of a cyst nematode acetylcholinesterase gene using Caenorhabditis elegans as a heterologous system.[J]. International journal for parasitology, 2009, 39(7):849-858. doi: 10.1016/j.ijpara.2008.12.007 URL
[15]	熊玉芬, 王暄, 梁中伟, 等. 松材线虫乙酰胆碱酯酶基因Bx-ace-2的克隆和序列分析[J]. 南京农业大学学报, 2010, 33(4):42-48.
[16]	KANG J S, LEE D W, KOH Y H, et al. A soluble acetylcholinesterase provides chemical defense against xenobiotics in the pinewood nematode[J]. Plos one, 2011, 6(4):e19063. doi: 10.1371/journal.pone.0019063 URL
[17]	KANG J S, LEE D W, CHOI J Y, et al. Three acetylcholinesterases of the pinewood nematode, Bursaphelenchus xylophilus: insights into distinct physiological functions[J]. Molecular & biochemical parasitology, 2011, 175(2):154-161.
[18]	张明, 李盾, 陈仪本, 等. 乙酰胆碱酯酶分子生物学研究进展[J]. 农药, 2006(1):8-11.
[19]	OPPERMAN C H, CHANG S. Nematode acetylcholinesterases: molecular forms and their potential role in nematode behavior[J]. Parasitology today, 1992, 8(12):406. pmid: 15463554
[20]	RC A, LEI Z A, YC A, et al. Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita[J]. Experimental parasitology, 2017, 176:75-81. doi: 10.1016/j.exppara.2017.01.008 URL
[21]	JOANA C C, CATHERINE J L, HOWARD J A, et al. Functional characterisation of a cyst nematode acetylcholinesterase gene using Caenorhabditis elegans as a heterologous system[J]. International journal for parasitology, 2009, 39(7):849-858. doi: 10.1016/j.ijpara.2008.12.007 URL
[22]	丁中, 彭德良, 高必达. 线虫乙酰胆碱酯酶研究进展[J]. 植物保护, 2008(3):18-21.
[23]	CLELAND T A. Inhibitory glutamate receptor channels[J]. Molecular neurobiology, 1996, 13(2):97-136. pmid: 8938647
[24]	LI B W, RUSH A C, WEIL G J. High level expression of a glutamate-gated chloride channel gene in reproductive tissues of Brugia malayi may explain the sterilizing effect of ivermectin on filarial worms[J]. International journal for parasitology: drugs and drug resistance, 2014, 4(2).

年份	活性成分	开发公司	类别/来源	其他
2021	三氟咪啶酰胺	科迪华	磺胺类	含氟杀线虫剂
2020	cyclobutrifluram	先正达	新型烟酰胺类	含氟杀线虫剂
2019	三氟杀线酯	山东中农联合生物科技股份有限公司	三氟丁烯类化合物	含氟杀线虫剂;具有自主知识产权
2018	Vydate杀线威	杜邦	氨基甲酸酯类	1974年开发
2017	Tioxazafen	孟山都	二取代二唑类化合物	由于引发皮疹,取消登记
2014	氟噻虫砜	安道麦	杂环氟代砜类	含氟杀线虫剂
2010	氟吡菌酰胺	拜耳	吡啶乙基苯甲酰胺类	含氟杀线虫剂;第一个作用于琥珀酸脱氢酶

年份	活性成分	开发公司	类别/来源	其他
2021	三氟咪啶酰胺	科迪华	磺胺类	含氟杀线虫剂
2020	cyclobutrifluram	先正达	新型烟酰胺类	含氟杀线虫剂
2019	三氟杀线酯	山东中农联合生物科技股份有限公司	三氟丁烯类化合物	含氟杀线虫剂;具有自主知识产权
2018	Vydate杀线威	杜邦	氨基甲酸酯类	1974年开发
2017	Tioxazafen	孟山都	二取代二唑类化合物	由于引发皮疹,取消登记
2014	氟噻虫砜	安道麦	杂环氟代砜类	含氟杀线虫剂
2010	氟吡菌酰胺	拜耳	吡啶乙基苯甲酰胺类	含氟杀线虫剂;第一个作用于琥珀酸脱氢酶

作用机制	代表杀线虫剂品种	杀虫剂/杀菌剂作用机制组别
神经：乙酰胆碱酯酶抑制剂	氨基甲酸酯类：涕灭威、克百威、杀线威等有机磷类：噻唑膦、甲拌磷等	IRAC：1A,1B
神经：谷氨酸门控氯离子通道变构调节剂	阿维菌素	IRAC：6
呼吸：线粒体复合体II电子传递抑制剂或琥珀酸辅酶Q还原酶抑制剂	氟吡菌酰胺、cyclobutrifluram	FRAC：7
脂质合成、生长调控,乙酰辅酶A羧化酶抑制剂	螺虫乙酯	IRAC：23
未知或不确定作用机制的化合物	氟噻虫砜、Tioxazafen、三氟咪啶酰胺、糠醛、异菌脲
未知或不确定作用机制的化合物：假定是多位点抑制剂	熏蒸剂：卤代烃类,异氰酸甲酯类等	IRAC：8

作用机制	代表杀线虫剂品种	杀虫剂/杀菌剂作用机制组别
神经：乙酰胆碱酯酶抑制剂	氨基甲酸酯类：涕灭威、克百威、杀线威等有机磷类：噻唑膦、甲拌磷等	IRAC：1A,1B
神经：谷氨酸门控氯离子通道变构调节剂	阿维菌素	IRAC：6
呼吸：线粒体复合体II电子传递抑制剂或琥珀酸辅酶Q还原酶抑制剂	氟吡菌酰胺、cyclobutrifluram	FRAC：7
脂质合成、生长调控,乙酰辅酶A羧化酶抑制剂	螺虫乙酯	IRAC：23
未知或不确定作用机制的化合物	氟噻虫砜、Tioxazafen、三氟咪啶酰胺、糠醛、异菌脲
未知或不确定作用机制的化合物：假定是多位点抑制剂	熏蒸剂：卤代烃类,异氰酸甲酯类等	IRAC：8