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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (2): 6-11.doi: 10.11924/j.issn.1000-6850.2012-1803

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸭IGF-IR基因部分序列克隆、鉴定及在胚胎期胸肌组织中的差异表达分析

束婧婷 单艳菊 胡艳 宋迟 宋卫涛 朱文奇 徐文娟 陈文峰 李慧芳   

  • 收稿日期:2012-05-10 修回日期:2012-06-21 出版日期:2013-01-15 发布日期:2013-01-15
  • 基金资助:
    国家自然科学基金“鸭胚胎期骨骼肌生长发育及调控机理的研究”(31172194)；江苏省科技支撑计划项目“苏邮1号蛋鸭配套技术创新研究”(BE2011329)；现代农业品种创新项目“高邮鸭配套系的创新选育与示范应用”(CX(11)1030)；江苏省家禽科学研究所青年基金项目“IGFs系统对鸭胚胎期胸肌生长发育的调控”(JQ201103)。

Cloning, Sequence Identification and Expression Analysis of IGF-IR in Breast Muscle at Different Embryonic Period in Ducks

  • Received:2012-05-10 Revised:2012-06-21 Online:2013-01-15 Published:2013-01-15

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摘要： 为了克隆分析鸭IGF-IR基因部分的cDNA序列，并研究该基因的组织表达规律。通过同源性比对，克隆了IGF-IR基因cDNA部分片段，并运用生物信息学方法分析了不同物种序列进化关系，运用荧光绝对定量PCR技术检测了IGF-IR mRNA在肉用型品种高邮鸭和蛋用型品种金定鸭胚胎期不同发育时期胸肌中的表达。序列分析结果表明：克隆得到的鸭IGF-IR基因cDNA270 bp片段长度，其核苷酸序列与鸡、人、小鼠、大鼠、猪、牛的IGF-IR基因相应核苷酸序列的同源性分别为95%、79%、78%、77%、78%、76%；荧光绝对定量结果表明：IGF-IR基因在胸肌中呈“波浪形”表达，在21胚龄表达量最高，与17、25、27胚龄和7日龄差异显著(P<0.05)；IGF-IR基因在品种间表达量除21胚龄时差异显著外(P<0.05)，其余各胚龄差异均不显著(P>0.05)，表明IGF-IR mRNA的表达在所研究品种胚胎期的胸肌中相对稳定。

关键词: 微肥, 微肥

Abstract: The present experiment was conducted to obtain and analyze partial cDNA sequence of duck Insulin- like growth factor I receptor gene (IGF-IR), and to study the tissue expression characterization of IGF-IR mRNA in ducks. Partial sequence of IGF-IR cDNA was cloned by homologous alignment analysis, the sequence was analyzed by bioinformatics method, and its absolute mRNA expression was detected by quantitative RT-PCR in breast muscle at different embryonic period meat-type duck breed-Gaoyou ducks and egg-type duck breed-Jinding ducks. Sequence analysis showed that the 270 bp of CDS region of duck IGF-IR gene shared 95% , 79% , 78% , 77% , 78% , 76% identity with those of Gallus gallus, Homo sapiens, Mus -musculus, Rattus novegicus, Sus domesticus and Bos Taurus, respectively. The results of quantitative RT-PCR indicated that the expression of duck IGF-IR showed“wave”trend at different embryonic period, the highest level appeared at 21 embryo age, which was significant higher than that of 17, 25, 27 embryo age and 7 day-old (P<0.05). The expression level of IGF-IR mRNA between breeds was relatively stable; the difference was significant at 21 embryo age (P<0.05), and the differences were not significant at other embryo age (P>0.05).