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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (11): 22-26.doi: 10.11924/j.issn.1000-6850.2012-1962

所属专题： 生物技术； 马铃薯； 园艺

• 生物技术科学 • 上一篇    下一篇

高氰和低氰木薯生氰葡萄糖苷酶Linamarase基因的组织表达差异分析

付莉莉,沈培峰,谭德冠,孙雪飘,张家明   

  1. 中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所
  • 收稿日期:2012-05-23 修回日期:2016-03-09 接受日期:2015-12-23 出版日期:2016-04-20 发布日期:2016-04-20
  • 通讯作者: 张家明
  • 基金资助:
    海南省自然科学基金“木薯淀粉磷酸化相关GWD基因的克隆及功能研究”(314123)；中央级公益性科研院所基本科研业务费专项“高乙醇转化率能源木薯新种质培育”(ITBB110501)。

Differential Expression of Linamarase Gene in Cassava Cultivars with Low Cyanide Content and High Cyanide Content

付莉莉,沈培峰,谭德冠,孙雪飘 and 张家明   

  • Received:2012-05-23 Revised:2016-03-09 Accepted:2015-12-23 Online:2016-04-20 Published:2016-04-20

摘要： 旨在降低或去除木薯的生氰糖苷，获得安全无毒木薯新品种。本研究以高氰和低氰5 个品种的木薯为材料，通过RT-PCR 方法，研究了生氰葡萄糖苷酶基因Linamarase 在木薯中的表达情况。木薯Linamarase 基因只在地上部分表达；5 个木薯品种，包括低氰和高氰品种，在块根中也均未检测到Linamarase 基因的表达。在地上部分中，顶芽的表达量要远远大于茎和成熟叶片的表达量，而且低氰和高氰品种在顶芽的表达量基本相同，但是低氰品种茎及成熟叶片中Linamarase 的表达量比高氰品种相对较高。木薯生氰糖苷酶Linamarase 在不同木薯品种不同组织的表达情况的不同，可以为筛选安全无毒木薯品种提供一个参考。

关键词: 多环芳烃(PAHs), 多环芳烃(PAHs)

Abstract: Cyanogenic glucosides were reduced or eliminated in order to obtain safe and non-toxic cassava varieties. In this study, the expression of a cyanogenic glucosidase gene was investigated in five cassava varieties (high-cyanide and low-cyanide) by RT-PCR. Linamarase was synthesized in the aboveground tissues of the plant and no expression was found in the underground part; the expression level of terminal bud on the aboveground part was much greater than the expression level of stems and mature leaves; no expression appeared in tuberous root of low cyanide and high cyanide varieties. No significant difference in expression was found on the terminal bud of high cyanide and low-cyanide cassava; however, higher expression appeared in leaves and stems of low cyanide cassava. The expression of linamarase in different varieties and different tissues of cassava was different. The study could provide certain reference for safe and non-poisonous cassava variety screening.