木薯淀粉分支酶SBEⅠ反义基因遗传转化木薯的研究

doi:10.11924/j.issn.1000-6850.2012-2104

中国农学通报 ›› 2013, Vol. 29 ›› Issue (9): 149-154.doi: 10.11924/j.issn.1000-6850.2012-2104

所属专题：生物技术；马铃薯

木薯淀粉分支酶SBEⅠ反义基因遗传转化木薯的研究

郭运玲孔华尹奇左娇黄启星贺立卡郭安平

收稿日期:2012-06-04 修回日期:2012-08-07 出版日期:2013-03-25 发布日期:2013-03-25
基金资助:
国家自然科学基金项目“木薯淀粉品质改良的转基因关键技术研究”(30960204)；现代农业产业技术体系项目“现代农业产业技术体系建设专项木薯岗位科学家经费”(nycytx-17)；国家重点基础研究发展计划(973)项目“重要热带作物木薯品质改良的基础研究”(2010CB126605)。

Genetic Transformation of Cassava with Its SBEⅠ Antisense Gene

Received:2012-06-04 Revised:2012-08-07 Online:2013-03-25 Published:2013-03-25

摘要/Abstract

摘要： 旨在获得转淀粉分支酶反义SBEⅠ基因的‘华南木薯8号’转基因植株，为利用转基因技术改良木薯淀粉品质打下基础。在建立了木薯从胚状体子叶到完整植株的再生体系的基础上，用块根特异表达启动子Sporamin驱动的木薯淀粉分支酶SBEⅠ反义基因，通过农杆菌介导法对‘华南木薯8号’进行遗传转化。共接种‘华南木薯8号’子叶517块，获得7株生长良好的转化再生植株，转化再生频率达到1.35%。经PCR检测，其中5株转化再生植株扩增出目的条带，初步证实木薯淀粉分支酶SBEⅠ反义基因已整合进了‘华南木薯8号’基因组中。通过农杆菌介导法可以将淀粉分支酶SBEⅠ反义基因导入到‘华南木薯8号’基因组中，获得了5株转基因植株。

关键词: 丰都县沿江地区, 丰都县沿江地区

Abstract: This study aimed to gain transgenic‘SC-8’cassava with the transformation of starch branching enzyme (SBEⅠ) antisense gene, and provide basis for using transgenic technology in the improvement of cassava starch quality. Based on the cassava regeneration system which could take the embryoid cotyledon to complete plants, the SBEⅠantisense gene was transformed by Agrobacterium-mediated transformation and the tuber-specific promoter Sporamin was used. 517‘SC-8’cassava cotyledons were inoculated which resulted in 7 well grown regenerated plants with a transformation rate of 1.35%. By PCR analysis, the SBEⅠantisense gene products were amplified in 5 regenerated plants which could provide evidence for the combination of SBEⅠantisense gene in cassava genome. The SBEⅠantisense gene can be successfully transformed to‘SC-8’cassava by the Agrobacterium-mediated transformation method. 5 transgenic‘SC-8’cassava plants were obtained.

郭运玲孔华尹奇左娇黄启星贺立卡郭安平. 木薯淀粉分支酶SBEⅠ反义基因遗传转化木薯的研究[J]. 中国农学通报, 2013, 29(9): 149-154.

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木薯淀粉分支酶SBEⅠ反义基因遗传转化木薯的研究

Genetic Transformation of Cassava with Its SBEⅠ Antisense Gene

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