欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (6): 69-74.doi: 10.11924/j.issn.1000-6850.2012-2693

所属专题： 油料作物

• 农学 农业基础科学 • 上一篇    下一篇

田间施加AM菌剂对大豆生长效应的影响

宋福强 程蛟 常伟 王倡宪 孔祥仕 王健   

  • 收稿日期:2012-08-02 修回日期:2012-09-03 出版日期:2013-02-25 发布日期:2013-02-25
  • 基金资助:
    国家自然科学基金资助项目“丛枝菌根对阿特拉津污染土壤修复机制研究”(31270535)，“AM真菌与紫穗槐形成菌根过程中共生相关蛋白的研究”(31070576)；黑龙江大学高层次人才支持计划项目“土壤障碍因子的生态修复”(Hdtd2010-12）；黑龙江省高等学校科技创新团队支持项目“农业微生物发酵技术”。

The Impact of AM Fungi on Soybean Growth with AM Inoculum Addition in Field

  • Received:2012-08-02 Revised:2012-09-03 Online:2013-02-25 Published:2013-02-25

PDF (PC)

764

摘要： 为进一步揭示在大田条件下施加丛枝菌根(AM)菌剂对作物生长的影响，以大豆为研究对象，田间播种时分别施加摩西球囊霉(Glomus mosseae，GM)和根内球囊霉(Glomus intraradices，GI)2种AM菌剂，以施加化肥处理(F)和既不施加菌剂也不施加化肥(CK)做为对照，研究AM菌剂对大豆生物量、根瘤数和土壤酶活的影响。结果表明：2种AM真菌在田间条件下都能侵染大豆根系形成菌根，GM处理的大豆菌根侵染率最高达到86.6%，GI处理的菌根侵染率最高达到78.3%。施加2种AM菌剂可显著提高大豆植株的生物量，GM、GI处理的大豆产量与CK相比分别提高了7.38%和3.88%。施加AM菌剂的大豆根瘤数显著高于CK处理，说明AM真菌能促进大豆根瘤的形成，从而提高大豆的固氮能力。施加AM菌剂可显著提高大豆根际土壤脲酶和过氧化酶的活性，且在大豆分枝期酶活性达到最大。另外，施加化肥能抑制脲酶的活性，使土壤中脲酶活性降低，而对过氧化氢酶活性的影响不显著。该研究结果为AM菌剂在田间推广应用奠定了理论基础。

关键词: 村落变迁, 村落变迁

Abstract: Taking fertilization group (F) and no-inoculation-fertilization group (CK) as control, soybean were inoculated when sowing with Glomus mosseae (GM) and Glomus intraradices (GI) respectively, the impact of arbuscular mycorrhiza (AM) fungi on soybean biomass, the amount of soybean rhizobium, soil enzyme activity and so on were investigated to further reveal the influence of AM inoculum on crops in field. The results showed that, soybean could be colonized by and form mycorrhiza with both the two AM fungi strains, of which the colonization percentage with GM inoculation was up to 86.6% of the highest, while 78.3% with GI. And both the two AM fungi could dramatically elevate soybean biomass by 7.38% with GM and 3.88% with GI inoculation compared to CK. In addition, the amounts of soybean rhizobium with AM fungi inoculation exceed significantly the two control groups as well, suggesting AM fungi could promote root nodule formation which in turn enhanced soybean nitrogen-fixing ability. Beside, the activities of soil catalase and urease increased significantly with AM inoculum addition and reached their top at branching stage. However, fertilization had a certain inhibitory effects on soil urease activity decreasing its activity in soil, while had no significant effect on soil catalase activity.