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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (1): 24-28.doi: 10.11924/j.issn.1000-6850.2012-2903

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

红花檵木CHS基因的克隆与序列分析

许威 于晓英 陈己任 符红艳   

  • 收稿日期:2012-08-23 修回日期:2012-10-21 出版日期:2013-01-05 发布日期:2013-01-05
  • 基金资助:
    国家自然基金面上项目“DREB和XET基因聚合表达的分子基础“(31071826)；湖南省高等学校科学研究项目重点项目“红花檵木叶色高温返青的分子机理研究“(10A057)。

Cloning and Sequence Analyzing of Chalcone Synthase Gene in Loropetalum chinense var. rubrum

  • Received:2012-08-23 Revised:2012-10-21 Online:2013-01-05 Published:2013-01-05

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摘要： 查尔酮合酶(chalcone synthase, CHS)是进入类黄酮和花色素苷次生代谢的第1个关键酶。根据植物查尔酮合成酶保守区序列设计引物，以红花檵木(Loropetalurn chinense var. rubrum)大叶红的嫩叶为材料，用RT-PCR方法，分离得到了一个查尔酮合成酶基因的cDNA（GenBank登录号为JQ609678），将该基因命名为LcvrCHS1。该序列长927 bp，编码232个氨基酸残基。其核苷酸序列与GenBank已登录的同样来源的核桃、山茶属植物CHS序列同源性达83%，与其他科植物（绣球花、葡萄、桃、马铃薯、甘草、领春木属）CHS序列同源性也达到80%以上；其编码的氨基酸序列与山茶属、葡萄、鳄梨、洋梨、沙梨、映山红CHS基因编码的氨基酸序列同样具有高度同源性，同源性高达98%。

关键词: 灌溉制度, 灌溉制度

Abstract: Chalcone synthase (chalcone synthase, CHS) is the key enzyme that catalyzes the first step in flavonoids biosynthesis and anthocyanins secondary metabolites. A full-length cDNA encoding CHS was cloned from the young leaves of Loropetalurn chinense var. rubrum by RT-PCR using specific primers based on the highly conserved sequences of plant CHS that had already known. Blast search revealed that it was a new gene, and was named as LcvrCHS1 (GenBank accession: JQ609678). The sequence was 927 bp, encoding 232 amino acid residues. It had 83% sequence homology with walnut and camellia that had been logged in GenBank; with other genus plants (hydrangea, grapes, peaches, potatoes, licorice, Euptelea genus), CHS sequence homology was also more than 80%; with other plants (camellia, grapes, avocados, bartlett pear, sand pear, azalea), CHS sequence also had high homology, up to 98% homology.