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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (4): 57-61.doi: 10.11924/j.issn.1000-6850.2012-2985

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

生姜中抗黑曲霉活性内生细菌的筛选与鉴定

刘娜 王琨   

  • 收稿日期:2012-09-03 修回日期:2012-10-18 出版日期:2013-02-05 发布日期:2013-02-05
  • 基金资助:
    河南省教育厅科学技术研究项目 “防霉型内生菌资源利用及绿色防霉剂的研究” (12B180039)。

Isolation and Identification of Antagonistic Ginger Endophytic Bacteria for Aspergillus niger

  • Received:2012-09-03 Revised:2012-10-18 Online:2013-02-05 Published:2013-02-05

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摘要： 为了找到一株具有安全、高防霉性特点的内生菌，以解决高水分粮食储藏时使用化学防霉剂的安全、成本问题。试验使用70%乙醇、10%次氯酸钠、5%石碳酸对生姜进行表面灭菌，使用研磨液梯度稀释结合平板划线法从生姜中分离到13株内生细菌。通过菌体对峙试验得到1株对黑曲霉具有明显抑制作用的内生细菌JT716。结合形态学特征、生理生化试验以及16S rDNA同源性分析鉴定该菌为荧光假单胞菌。

关键词: 灰色局势决策, 灰色局势决策

Abstract: In order to find a safe, mildew resistance endophytes to solve the problems of safe and cost of the use of chemical fungicides. Ginger was surface sterilized by using 70% ethanol, 10% sodium hypochlorite and 5% phenol. 13 endophytes were isolated from ginger by using gradient dilution and streak plate method. After repetitive co-culture on PDA plates, results showed that the growth of Aspergillus niger could be strongly inhibited by JT716. According to the morphology characteristics, physiology and biochemistry tests, the comparison of 16S rDNA sequence, JT716 was similar to Pseudomonas fluorescens.