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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (15): 183-187.doi: 10.11924/j.issn.1000-6850.2012-3674

所属专题： 生物技术； 植物保护

• 植物保护 农药 • 上一篇    下一篇

11株侧柏内生真菌的农药活性及分子生物学鉴定

王静 易晓华 魏艳 郝双红   

  • 收稿日期:2012-11-12 修回日期:2012-12-24 出版日期:2013-05-25 发布日期:2013-05-25
  • 基金资助:
    具除草活性的侧柏内生真菌J46的生物学特性及其活性成分的分离鉴定，山东省自然科学基金（联合专项）

Pesticide Activity and Molecular Biology Identification of 11 Strains of Endophytic Fungi from Oriental Arborvitae

  • Received:2012-11-12 Revised:2012-12-24 Online:2013-05-25 Published:2013-05-25

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摘要： 分别采用生长速率法和种子萌发法测定了11株侧柏内生真菌J46、J77、J80、J85、J86、J94、Y51、Y52、Y57、Y125、Y127发酵液对苹果腐烂病菌、番茄灰霉病菌等12种植物病原菌的抑菌活性及对反枝苋种子的除草活性。结果表明，有10株内生真菌对至少一种植物病原菌的抑制率大于50%；其中Y52、J94、Y127、J77、J46等5种内生真菌对苹果腐烂病菌的抑制率高于70%，J94、Y52、J77、J46等4种内生真菌对番茄灰霉病菌抑制率高于70% 。内生真菌对反枝苋种子幼根的生长表现出较强抑制作用，抑制率在70%以上的有8株。对筛选出的8株具有较好抑菌或除草活性的侧柏内生真菌进行了ITS 序列系统发育分析，确定其均为链格孢属链格孢菌（Alternaria alternaria）。

关键词: 反演模型, 反演模型

Abstract: The antifungal and herbicidal activity of 11 strains of endophytic fungi ( J46, J77, J80, J85, J86, J94, Y51, Y52, Y57, Y125, Y127 ) from Oriental Arborvitae against the mycelium growth of 12 kinds of plant pathogenic fungi and seed germination of Amranthus retroflexus were tested respectively. The results showed that the inhibition rate of 10 strains of endophytic fungi were more than 50% at least to one pathogenic fungus. There were 5 strains of endophytic fungi ( Y52, J94, Y127, J77 and J46) whose inhibition rate against Valsa mali were more than 70%, and 4 strains of endophytic fungi (J94, Y52, J77 and J46) whose inhibition rate against Botrytis cinerea were more than 70%. The endophytic fungi displayed strong inhibition to the radicle of A. retroflexus, in which inhibition rate of 8 strains were more than 70%. ITS sequence was determined to classify the 8 strains of endophytic fungi with good antifungal or herbicidal activity, and it showed that they were all Alternaria alternaria.

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