欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (10): 18-21.doi: 10.11924/j.issn.1000-6850.2012-3758

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

‘秋天火焰’槭类黄酮糖基转移酶（UFGT）基因的克隆及序列分析

尹燕雷 苑兆和 招雪晴 冯立娟   

  • 收稿日期:2012-11-19 修回日期:2012-12-24 出版日期:2013-04-05 发布日期:2013-04-05
  • 基金资助:
    两个优选槭树品种叶色表达相关基因CHI和UFGT的分离、鉴定和克隆

Cloning and Sequence Analysis of a UFGT Gene Fragment from maple cultivar ‘Autumn Blaze’

  • Received:2012-11-19 Revised:2012-12-24 Online:2013-04-05 Published:2013-04-05

PDF (PC)

891

摘要： UFGT（类黄酮糖基转移酶）是植物花色苷生物合成途径的最后一个酶。以槭树‘秋天火焰’秋季变色期叶片总RNA为模板，设计简并引物，采用RT-PCR方法，扩增出一条长397bp的cDNA片段，序列分析结果表明，该cDNA片段与GenBank中登录的荔枝、柑橘柚等物种的氨基酸同源性在70%左右，可知克隆到的核苷酸片段为槭树叶片UFGT基因的cDNA片段，Genbank登录号为KC140229。

关键词: 流失风险, 流失风险

Abstract: UFGT（UDP glucose –flavonoid-3-o-glycosyltranferase）is the last key enzyme in the biosynthesis of anthocyanins. Total RNA was isolated from autumn leaves of Acer×freemanii ‘Autumn Blaze’. Using degenerate primer, a new cDNA fragment of 397 bp was amplified by RT-PCR. Sequence analysis demonstrated that the deduced amino acid sequence shared above 70% identity with Litchi chinensis, Citrus x paradisi, etc. These results suggested that the cDNA fragment cloned in this study was from UFGT gene of maple. The accession number in GenBank is KC140229.

中图分类号: