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中国农学通报 ›› 2013, Vol. 29 ›› Issue (12): 212-216.doi: 10.11924/j.issn.1000-6850.2012-3913

所属专题: 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

大米蛋白质含量近红外光谱检测模型研究

刘明博 唐延林 李晓利 李玉鹏 凌志刚   

  • 收稿日期:2012-12-03 修回日期:2013-01-03 出版日期:2013-04-25 发布日期:2013-04-25
  • 基金资助:
    国家自然科学基金;国家自然科学基金;国家自然科学基金

Study on NIR Spectral Detection Model of Rice Protein Content

  • Received:2012-12-03 Revised:2013-01-03 Online:2013-04-25 Published:2013-04-25

摘要: 探索建立一种有效的大米蛋白质含量近红外光谱检测模型,并寻找1100~2500nm波段中预测大米蛋白质含量的有效波长。采用面积归一化(Area Normalization)方法进行光谱预处理。用主成分回归方法建立回归模型,用Martens不确定性检验方法选择有效波长。发现利用主成分分析可以较好地区分出不同种类的米粉,样品在主成分上的得分可以作为鉴别米粉种类及品质的依据。基于全部波长建立的回归模型,训练集r=0.9923,RMSE=0.0747。交叉验证的结果r=0.9399,RMSE=0.2103。预测集r=0.9364,RMSE=0.1607。基于有效波长建立的回归模型,训练集r=0.9899,RMSE=0.0854。交叉验证结果r=0.9437,RMSE=0.2004。预测集r=0.9079,RMSE=0.1796。使用近红外光谱分析技术检测大米蛋白质含量是可行的,采用Martens不确定性检验方法选择有效波长,并利用有效波长预测大米蛋白质含量也是可行的。

关键词: 隶属度函数, 隶属度函数

Abstract: An effective NIR spectral detection model of rice content was explored, and effective wavelengths for the detection of rice protein content between 1100~2500nm were found. Area normalization method was used to preprocess the spectrum. Principal components regression (PCR) was used to build the regression model, effective wavelengths were selected by Martens’ uncertainty test. We found that different kinds of rice flour can be distinguished well using principal components analysis. Varieties and quality of rice can be identified according to the sample scores along principal components. Regression model based on all wavelengths has the regression coefficient r=0.9923, RMSE=0.0747 in training set, r=0.9399, RMSE=0.2103 in cross validation set, and r=0.9364, RMSE=0.1607 in prediction set. Regression model based on effective wavelengths has the regression coefficient r=0.9899, RMSE=0.0854 in training set, r=0.9437, RMSE=0.2004 in cross validation set, and r=0.9079, RMSE=0.1796 in prediction set. It is feasible to detect rice protein content using NIR spectroscopy. It is also feasible to select effective wavelengths using Martens’ uncertainty test and to detect rice protein content using these effective wavelengths.

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