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中国农学通报 ›› 2013, Vol. 29 ›› Issue (22): 178-182.doi: 10.11924/j.issn.1000-6850.2012-4167

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

黄瓜幼苗对NaCl胁迫的生理响应

刘凤兰 杜新民 秦爱萍   

  • 收稿日期:2012-12-27 修回日期:2013-01-25 出版日期:2013-08-05 发布日期:2013-08-05
  • 基金资助:
    黄土高原旱薄地区黍稷抗旱耐瘠根土系统研究

Different physiological responses of cucumber seedlings to NaCl stress

  • Received:2012-12-27 Revised:2013-01-25 Online:2013-08-05 Published:2013-08-05

摘要: 土壤盐渍化是人类面临的生态危机之一,它严重影响植物的正常生长。本文采用向1/2 Hoagland营养液中按一定比例添加中性盐(NaCl)模拟盐胁迫的方式,调查了NaCl胁迫对黄瓜幼苗生长的影响。结果表明:(1)NaCl胁迫下,黄瓜幼苗的株高、叶面积、地上部分鲜重、总根数、总根长及根系鲜重均呈下降趋势,除地上部分鲜重外,其余均随NaCl浓度的增加而明显下降。(2)NaCl胁迫下,黄瓜幼苗的叶绿素含量明显提高,但随NaCl浓度的增加叶绿素含量呈下降态势。(3) NaCl胁迫能明显降低黄瓜幼苗根苗SOD、POD、CAT活性,且地下部分SOD与CAT酶活性下降幅度大于地上部分。(4)黄瓜根苗的MDA含量随NaCl有胁迫浓度的增加而明显增加。(5)盐胁迫下,由于黄瓜幼苗根苗的抗氧化酶活性下降,从而使其膜质过氧化加重,最终导致其根苗生长受到明显的抑制。

关键词: 可视化检测, 可视化检测

Abstract: Soil salinization iw one of the issues that hunman being is facing, which was gravely effected plants to grow normally. An experiment using NaCl to induce 1/2 Hoagland water stresses was carried out to evaluate the effect on root and seedling growth of cucumber bean under slat-stresses , The result showed that (1) NaCl stress decrased plant height, leaf area, aerial parts of the fresh weight, total root number, total root length and root fresh wight of cucumber seedling. With the NaCl stress increasing, all of them are decreased except the aerial parts of fresh weight. (2) The chlorophyll content of cumcumber seedling increased remarkably under the NaCl stress. (3) The NaCl stress reduces the activity of cumcumber seedling roots’ SOD, POD, CAT. In addition the decrease range of SOD and CAT on enzyme activity of under ground portion was more than that of aerial portion. (4) The MAD content of cumcumber seedling was abviously increasing due to the increasing of NaCl stress concentration. (5) As the declining of antioxidase activity of cumcumber seedling roots, the membrance lipid peroxidation was enhanced, this ultimately lead to the inhibition of growth of cumcumber seedling root significantly.

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