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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (16): 7-11.doi: 10.11924/j.issn.1000-6850.2013-0017

• 林学 园艺 园林 • 上一篇    下一篇

彰武松腋芽诱导研究

范岳霞 袁文华 孔冬梅   

  • 收稿日期:2013-01-05 修回日期:2013-01-24 出版日期:2013-06-05 发布日期:2013-06-05
  • 基金资助:
    山西省林业厅林业关键技术攻关项目

Axillary Bud Induction in Pinus densiflora var. zhangwuensis

  • Received:2013-01-05 Revised:2013-01-24 Online:2013-06-05 Published:2013-06-05

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摘要： 为了探讨彰武松组培快繁的可行性，以彰武松封顶芽为外植体进行培养，研究灭菌方法、培养基成分以及植物生长调节物质等对其腋芽诱导的影响。结果表明：外植体用70%的乙醇处理30 s后用0.1%的HgCl2灭菌5 min，在添加BA 2~5 mg/L和蔗糖30 g/L的WPM培养基上，腋芽诱导率达50%~70%，NAA对腋芽诱导无促进作用。通过在添加和不添加植物生长调节物质(PGR)的WPM培养基中交替继代培养可以实现芽丛伸长与增殖。

关键词: 繁殖性状, 繁殖性状

Abstract: In order to explore the feasibility of propagating Pinus densiflora var. zhangwuensis by tissue culture, the dormant shoot buds of the species were used as explants to investigate the influence of sterilization methods, medium composition and plant growth regulators on axillary bud induction. The results showed that: the most effective sterilization treatment was rinsing the explants in 70% ethanol for 30 s following with 0.1% HgCl2 for 5 min. The optimal medium for axillary bud induction was WPM containing 2-5 mg/L BA and 30 g/L sucrose (shoot induction 50%-70%). NAA didn’t promote axillary bud induction. The new axillary shoots alternately subcultured in WPM with and without PGR elongated and proliferated well.

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