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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (36): 313-318.doi: 10.11924/j.issn.1000-6850.2013-0301

• 生物技术科学 • 上一篇    下一篇

工业大麻内生真菌菌群结构及其在植株内的空间分布特征

金蕊 杨明挚 刘飞虎   

  • 收稿日期:2013-01-28 修回日期:2013-03-18 出版日期:2013-12-25 发布日期:2013-12-25
  • 基金资助:
    国家麻类产业技术体系建设专项资金

Composition and distribution of endophytic fungi in Cannabis sativa L.

  • Received:2013-01-28 Revised:2013-03-18 Online:2013-12-25 Published:2013-12-25

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摘要： [目的]研究旨在探讨不同样地和不同部位大麻组织中内生真菌的多样性及其分布特征，[方法][结果]从4个不同样地大麻（云麻1号）的植株不同部位组织中共分离得到内生真菌531株，其中茎分离出138株，叶分离出393株。经形态学观察和ITS序列分子鉴定发现其分属于29个属，其中茎22个属，叶24个属。有18个属普遍存在于大麻茎和叶中，其中炭疽菌属是大麻的优势菌属，优势度为40.11%。大麻茎部的内生真菌分离率显著低于叶部，接近地面部分的大麻茎叶组织内生真菌分离率最高。4个样地大麻内生真菌多样性指数在1.84到2.46之间，不同样地中大麻内生真菌组成的相似系数普遍较高，同一样地中大麻茎与叶的相似性系数较小。[结论]结果表明，工业大麻（云麻1号）内生真菌多样性偏低、宿主专一性较小。

关键词: 地衣芽孢杆菌, 地衣芽孢杆菌

Abstract: [Objective] This study is to research for the composition and distribution of endophytic fungi in Yunnan hemp 1. [Method][Result]531 endophytic fungi strains (138 from stem; 393 from leaf) were isolated from stem and leaf samples of industrial hemp (Yunnan hemp 1) collected from different sites, and identified by morphological and molecular methods. The isolated endophytic fungi were divided into 29 genera (22 genera from stem; 24 genera from leave),of which 18 genera were found both in stem and leaf. Colletotrichum was the dominant genus with dominance index 40.11%. The isolation rate of endophytic fungi in stem was evidently lower than that in leaf. Overall, the highest isolation rate was got in the samples from the basal part of hemp plants. The diversity index of endophytic fungi in hemp from the four sampling sites varied from 1.84 to 2.46. The similarity coefficients were high among all sampling sites, but they were low between stem and leaf from the same site. [Conclusion]The results showed that the host specificity and diversity of endophytic fungi in industrial hemp (Yunnan hemp 1) were low.