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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (25): 42-48.doi: 10.11924/j.issn.1000-6850.2013-0681

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

枫杨组织培养快繁技术研究

王春荣 毕君 张往祥 曹福亮   

  • 收稿日期:2013-03-13 修回日期:2013-05-17 出版日期:2013-09-05 发布日期:2013-09-05
  • 基金资助:
    逆境生态林树木种质优选与示范

Study on Rapid Micropropagation System of Pterocarya stenoptera C.DC. by Tissue Culture

  • Received:2013-03-13 Revised:2013-05-17 Online:2013-09-05 Published:2013-09-05

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摘要： 为了实现枫杨优良种源和变异个体的种质保存和快速繁殖，选用枫杨带腋芽半木质化茎段为外植体，进行组织培养技术研究。结果表明：枫杨外植体适宜消毒处理为75%酒精30 s+0.1% HgCl2 5 min，外植体接种成功率可达87.5%；枫杨愈伤增殖基本培养基以MS为宜，而试管芽苗增殖和根诱导DKW培养基明显优于MS、B5；试管芽苗增殖适宜培养基为(1.0~2.0) mg/L 6-BA+1.0 mg/L KT+(0.3~0.5) mg/L IBA，20~25天增殖4倍以上；根诱导以1/2 DKW+(0.5~0.7) mg/L NAA和1/2 DKW+0.5 mg/L NAA+0.2 mg/L IAA为宜，生根率82%以上。

关键词: 发芽势, 发芽势

Abstract: For germplasm conservation and rapid propagation of Pterocarya stenoptera C.DC., the stem-segment with axillary buds taken as explants to be studied for tissue culture system. The results showed that, the best sterilization method was that explants could be treated with 75% ethyl alcohol and 0.1% HgCl2 for 30 s and 5 min, respectively. The percentage of sterile explant could arrive at 87.5%. The best basic medium of callus proliferation was MS, but DKW was better than MS and B5 to be used in test-tube plantlet multiplication and root induction. It was suggested that DKW supplemented with (1.0-2.0) mg/L 6-BA+1.0 mg/L KT+(0.3-0.5) mg/L IBA to be desirable treatment for subculture and multiplication, with 4 times multiplication rate after a 20-25 days cultivation. The media with 1/2 DKW+(0.5-0.7) mg/L NAA or 1/2 DKW+0.5 mg/L NAA+0.2 mg/L IAA was suggested for the rooting induction. The rooting rate was above 82%.