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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (2): 13-17.doi: 10.11924/j.issn.1000-6850.2013-1620

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

抗冻剂和冷冻速率对蛋用种公鸡精液冷冻保存效果的影响

张凯 吴海云 张立昌 王稳 刘云海 倪和民 郭勇   

  • 收稿日期:2013-06-13 修回日期:2013-09-04 出版日期:2014-01-15 发布日期:2014-01-15
  • 基金资助:
    2012年现代农业产业技术体系北京市家禽创新团队营养与饲料功能研究室岗位专家(PXM2012-014207-000166)。

Effect of Cryoprotectant and Freezing Rate on Semen Cryopreservation of Breeder Cock

  • Received:2013-06-13 Revised:2013-09-04 Online:2014-01-15 Published:2014-01-15

摘要： 为提高蛋用种公鸡精液冷冻效果，本试验研究了抗冻剂和冷冻速率对海兰褐蛋用种公鸡精液冷冻效果的影响。试验一：采用的冷冻保护剂为二甲基乙酰胺(DMA)与二甲基亚砜(DMSO)，浓度分别是6%、10%、15%。结果显示：10%DMSO处理组冷冻解冻后精子总活力达到37.4%，复苏率达到50%，活率达到58.3%；试验二：比较了3组冷冻速率，其中，以速率为7℃/min，从5℃下降至-35℃，再以速率为9℃/min ，从-35℃下降至-120℃处理组，冷冻解冻后精子总活力达到44.3%，总复苏率达到了53%，畸形率为41.9%，顶体完整率为42.9%；试验三：通过人工输精来评价其最终冷冻解冻效果（冻精解冻后的平均受精率为24.9%）。综上所述：在本试验条件下，以10%的DMSO作为冷冻保护剂，以7℃/min，从 5℃下降至-35℃，再以9℃/min，从-35℃下降至-120℃处理过的精子冷冻解冻效果最好。

关键词: 增效作用, 增效作用

Abstract: In this study, it was aimed to improve the freezing effect of the frozen semen from Hy-Line Brown breeder cock. Therefore, the effects of cryoprotectant and freezing rate on their semen cryopreservation were also investigated respectively. In the first experiment, both DMA and DMSO were applied as cryoprotectants, and their concentration were 6%, 10%, 15% respectively. The results showed as following: in the group of DMSO (10%), the percentage of total motility of sperm was 37.4%，the percentage of total recovery rate of sperm was 50% and the viability rate of sperm was 58.3%. In the second experiment, three cooling rates were designed totally and compared with one another. The results showed as following: in the best cooling rate group, total motility rate of sperm reached to 44.3%, recovery rate reached to 53%, and morphologically abnormal sperm rate and acrosome integrity rate were 41.9% and 42.9% respectively. Finally, the effect of frozen Breeder cock semen was also evaluated by their artificial insemination (24.9%). Collectively, it can be concluded that combination of 10% DMSO with the cooling rate being treated by the temperature from 5℃ to -35℃at 7℃/min, and then from -35℃ to -120℃ at 9℃/min，initiate the best inseminated effect of their freezing semen.