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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (33): 358-362.doi: 10.11924/j.issn.1000-6850.2013-1921

所属专题： 生物技术； 园艺

• 植物保护 农药 • 上一篇    下一篇

桃褐腐病菌激活蛋白对草莓幼苗防御酶酶活性的影响

张蒙 刘正坪 魏艳敏 尚巧霞 刘素花 赵晓燕   

  • 收稿日期:2013-07-12 修回日期:2013-08-05 出版日期:2013-11-25 发布日期:2013-11-25
  • 基金资助:
    北京市教委资助项目;科研基地建设-科技创新平台-害虫综合防治创新平台建设;北京市属高等学校人才强教计划资助项目之学术创新团队计划项目

The effect of activator protein of Monilinia fructicola on defence enzyme activity in strawberry seedlings

  • Received:2013-07-12 Revised:2013-08-05 Online:2013-11-25 Published:2013-11-25

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摘要： 激活蛋白是从多种病原真菌中分离出的一种蛋白激发子，为了解桃褐腐病菌激活蛋白对植物防御酶的诱导作用，本实验通过沸水浴、离心等方法,从桃褐腐病菌（Monilinia fructicola）菌丝体中分离出激活蛋白的粗提物，并喷雾到草莓幼苗的叶片上，测定了部分防御酶和病程相关蛋白的活性变化。结果表明：经桃褐腐病菌激活蛋白处理后，0-18h内草莓叶片的苯丙氨酸解氨酶(PAL)活性呈曲线增高的趋势，18h达到最高值，最高值比对照增加40.7%； 0-6h内草莓叶片过氧化物酶(POD) 和多酚氧化酶（PPO）活性均呈急剧增高的趋势，6h达到最高值，其中POD活性比对照增加126.2%，PPO活性比对照增加62.9%。经激活蛋白处理后草莓叶片的β-1,3-葡聚糖酶、几丁质酶活性也有增高的趋势。

关键词: 建模, 建模

Abstract: Activator protein is a kind of protein elicitor extracted from many plant pathogenic fungi. In order to understand the resistance induction of activator protein of Monilinia fructicola, activator protein was extracted from M. fructicola mycelium by boiling-water-bath and centrifugation and sprayed on leaves of strawberry seedlings. The activity of some defence enzymes were detected and the results showed that the activity of PAL increased in 0 ~ 18 h, and reached the peak at 18h with a peak value 40.7% higher than control; the activity of POD and PPO increased rapidly in 0 ~ 6 h, and reached the peak at 6h with peak values of POD 126.2% higher than control and PPO 62.9% higher than control. The activity of β-1, 3-glucanase and exo-chitinase increased after treat with activator protein of M. fructicola.