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中国农学通报 ›› 2014, Vol. 30 ›› Issue (18): 166-170.doi: 10.11924/j.issn.1000-6850.2013-2594

• 农学 农业基础科学 • 上一篇    下一篇

引进菌株Bionectria ochroleuca对几种植物病原菌的拮抗效应

宋福强 王占斌 王倡宪 杜春梅 常伟   

  • 收稿日期:2013-10-07 修回日期:2013-11-01 出版日期:2014-06-25 发布日期:2014-06-25
  • 基金资助:
    黑龙江省对外科技合作项目 “生物杀菌剂Дизофунгин引进及应用” (WB10B107)。

Antagonistic Effects of the Introduced Fungus Bionectria ochroleuca on Several Plant Pathogenic Fungi

  • Received:2013-10-07 Revised:2013-11-01 Online:2014-06-25 Published:2014-06-25

摘要: 为挖掘对植物病害防治有效的生防菌株,研究了引进菌株Bionectria ochroleuca对几种植物病原真菌的拮抗效应,旨在对其推广应用奠定理论基础。引进菌株B. ochroleuca对植物病原真菌Alternaria solani、Botrytis cimerca、Fusarium oxysporum.sp cucumebrium Owen、Fusarium graminearum Sch1. f. sp vasinfectum (Atk.) Snyd&Hans.、Colletotrichum capsici、Fusrium moniliforme、Fusarium graminearum、Cercosporidium sofinum均有不同程度的抑制作用,平均抑菌带宽度在3.5~14.4mm范围内,且其发酵产物对病原菌菌丝的生长及孢子的萌发也具有很好的抑制效果,表明B. ochroleuca菌株能够分泌胞外抑菌物质。B. ochroleuca菌株发酵产物对不同病原真菌的抑制结果具有显著差异,特别是对F. oxysporum.sp cucumebrium Owen抑制效果最好,菌丝生长抑制率高达73.3%,孢子萌发相对抑制率高达78.2%,并使萌发孢子的芽管发生畸变。B. ochroleuca发酵产物能够显著降低病原真菌菌丝内的SOD、CAT保护酶活性(P<0.05),处理21h时,F. oxysporum.sp cucumebrium菌丝体内的SOD酶活仅是对照处理的28.8%,CAT酶活是其对照处理的34.8%。引进菌株B. ochroleuca作为植物病原真菌的生防菌株具有一定的应用潜力。

关键词: 难溶性磷, 难溶性磷

Abstract: In order to explore biological control strains for effectively preventing and controlling plant diseases, Bionectria ochroleuca was introduced and its antagonistic effects on several plant pathogenic fungi were investigated, aiming at laying a theoretical foundation for its widespread application. In our study, the introduced fungal B. ochroleuca exhibited a varying degree of inhibition on plant pathogenic fungi including Alternaria solani, Botrytis cimerca, Fusarium oxysporum. sp cucumebrium Owen, Fusarium graminearum Sch1. f. sp vasinfectum (Atk.) Snyd & Hans., Colletotrichum capsici, Fusrium moniliforme, Fusarium graminearum, and Cercosporidium sofinum, with average width of inhibition zone was at 3.5~14.4 mm. Besides, the fermentation product of B. ochroleuca also showed good inhibitory effects on hyphal growth and spore germination of the pathogens, implying B. ochroleuca could secrete some extracellular antibacterial substances. However, its fermentation product displayed significant difference in inhibitory effects regarding to different strains, especially, showed the best inhibitory effects on F. oxysporum. sp cucumebrium Owen, with hyphal growth inhibition rate up to 73.3% and relative inhibition rate of spore germination was 78.2%, and in addition, it could lead to the distortion of germ tube which was used for spore germination. Furthermore, the fermentation product of B. ochroleuca could dramatically decrease the activity of intracellular protective enzymes as SOD and CAT (P<0.05) in pathogenic fungal hyphae. After treated for 21 hours, the SOD activity within the hyphae of F. oxysporum. sp cucumebrium was just 28.8% of the control, and CAT was 34.8% of the control. The above results showed that the introduced fungus B. ochroleuca had a certain application potential of being used as a biological control strain for plant pathogenic fungi.