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中国农学通报 ›› 2013, Vol. 29 ›› Issue (24): 28-35.doi: 10.11924/j.issn.1000-6850.2012-2246

所属专题: 生物技术 玉米

• 农学 农业基础科学 • 上一篇    下一篇

低磷胁迫下不同基因型玉米对难溶性磷酸盐的吸收和利用

陶佩琳 刘寒寒 邓红 张可炜   

  • 收稿日期:2012-06-18 修回日期:2012-08-06 出版日期:2013-08-25 发布日期:2013-08-25
  • 基金资助:
    国家自然科学基金资助项目;山东省科技发展计划项目;山东省自然科学基金项目

Genotypic Variation of Maize in Uptake and Utilization of Sparingly Soluble Phosphate Under Low-P Stress

  • Received:2012-06-18 Revised:2012-08-06 Online:2013-08-25 Published:2013-08-25

摘要: 为了解低磷胁迫下不同基因型玉米对难溶性磷酸盐的吸收利用的差异,以不同磷效率玉米自交系SDKH502和SDLH502为材料,采用营养液培养和砂培的方法,研究不同磷处理对玉米幼苗的生长以及对难溶性磷酸钙、磷酸铁、磷酸铝吸收利用能力的影响。结果表明:正常供磷处理和低磷处理的玉米植株的根系分泌物对难溶性磷酸盐都具有一定的活化能力,低磷胁迫下玉米的根系分泌物对Al-P、Ca-P或Fe-P的活化能力高于正常供磷处理的植株。与足磷下相比,低磷下SDKH502的根系分泌物对Al-P、Ca-P、Fe-P的活化能力依次提高了69.28%、87.34%和51.83%,SDLH502则依次提高了165.67%、141.13%和6.35%。与足磷下相比,低磷下SDKH502和SDLH502的根系分泌的有机酸分别提高了26.96%和18.85%,pH分别降低了0.79、0.57个单位,SDKH502和SDLH502间存在显著差异。在低磷胁迫下,自交系SDKH502和SDLH502活化吸收难溶性磷酸盐能力的不同与SDKH502和SDLH502根系分泌有机酸和质子能力的差异有关。

关键词: 诱导抗性, 诱导抗性

Abstract: In order to investigate the genotypic variation of maize in uptake and utilization of sparingly soluble phosphate under Low-P stress, two inbred lines SDKH502 and SDLH502 of maize with different phosphorous use efficiency are used as materials. Sand pot and hydroponics experiments are carried out to study the effect of differences phosphorous supplying on growth of seedling, and the ability of mobilization and utilization for sparingly soluble phosphate (Al-P, Ca-P or Fe-P) of maize. The experimental results demonstrate that the root exudates can mobilize the sparingly soluble phosphates in the condition of normal phosphate supply and phosphate starvation, and phosphate starvation caused the improvement in mobilization of sparingly soluble phosphate (Al-P, Ca-P or Fe-P). Compared with normal phosphate supply condition, the ability of activating the sparingly soluble Al-P, Ca-P, Fe-P by root exudates of SDKH502 increased by 69.28%, 87.34% and 51.83%, while the SDLH502 enhanced by 165.67%, 141.13, and 6.35% under phosphate deficient condition. Beside that, the organic acids secretion from roots of SDKH502 and SDLH502 increased by 26.96% and 18.85%, pH value depressed by 0.79 and 0.57 units separately. There were remarkable differences between SDKH502 and SDLH502 in responding to phosphate starvation. Those results above showed that, the difference of the secretion of organic acids and proton might result in various mobilization and absorption ability of sparingly soluble phosphate between SDKH502 and SDLH502.