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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (36): 367-371.doi: 10.11924/j.issn.1000-6850.2013-1563

所属专题： 生物技术； 园艺

• 植物保护 农药 • 上一篇    下一篇

一种生物诱抗剂防治黄瓜白粉病效果及对诱导酶的影响

刘琴 吴毅歆 薛原 毛自朝 黄莲英 何月秋   

  • 收稿日期:2013-06-05 修回日期:2013-09-17 出版日期:2013-12-25 发布日期:2013-12-25
  • 基金资助:
    国家高技术研究发展计划（863计划

Effect of a Biological Resistance Inducer on Controlling Cucumber Powdery Mildew and Inducing Enzymes Activities

  • Received:2013-06-05 Revised:2013-09-17 Online:2013-12-25 Published:2013-12-25

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摘要： 为了明确生物诱抗剂（Biological Resistance Inducer ，BRI）对黄瓜白粉病的防治效果及抗性相关诱导酶的影响，进行了盆栽接种和诱导酶活性测定。结果表明，施用诱抗剂浓度越高和次数越多，防病效果越好。喷施诱抗剂10μg/mL、20μg/mL和100μg/mL 24h后，接种病菌72h后，防病效果分别达59.91%、85.02%和89.43%；每隔24h喷施100μg/mL1次、2次、3次和4次，72h后，防治效果分别达80.32%、98.23%、98.30%和100.00%。诱抗剂提高了黄瓜叶片中的POD、SOD、PAL和CAT等防御相关酶活性，至第5-7天仍然持很高水平，其中PAL活性至第9天仍达空白对照的4.37倍。

关键词: 产量, 产量

Abstract: To understand the effect of a biological resistance inducer(BRI) on controlling cucumber powdery mildew and inducing enzymes activities, the potted, inoculation and POD, SOD, PAL and CAT tests were proceeded. The results showed that the better control effect was the higher concentration and more sprays of BRI were used. The control effects of cucumber powdery mildew were 59.91%, 85.02% and 89.43% after 72 hours when 10μg/mL, 20μg/mL and 100μg/mL of BRI were sprayed onto cucumber seedlings before 24 hours when the pathogen was inoculated and 80.32%, 98.23%, 98.30% and 100.00% after 72 hours when 100μg/mL of BRI were sprayed 1, 2, 3 and 4 times before the pathogen was inoculated. BRI increased the activities of the defense-related enzymes, POD, SOD, PAL and CAT of cucumber leaf until 5-7 days after its spray, especially PAL activity was as 4.37 times high as the negative control without any sprays at the 9th day.