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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 186-189.doi: 10.11924/j.issn.1000-6850.2013-2704

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

绣球丁香扦插生根过程中内源激素变化

颜婷美 张安琪 王峰 李承秀 王郑昊 王长宪   

  • 收稿日期:2013-10-16 修回日期:2013-10-29 出版日期:2014-05-05 发布日期:2014-05-05
  • 基金资助:
    山东省农业良种项目 “泰山稀特树种木绣球、 五角枫等种质资源保护利用和品种创新研究” [鲁科农计字(2007)217号]。

Changes of Endogenous Hormones During Syringa microphylla Cutting

  • Received:2013-10-16 Revised:2013-10-29 Online:2014-05-05 Published:2014-05-05

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摘要： 为探究绣球丁香嫩枝扦插生根机理，建立高效的扦插繁殖技术体系，采用高效液相色谱法对其生根过程中吲哚乙酸(IAA)、赤霉素(GA3)、脱落酸(ABA)、玉米素(ZT) 4种内源激素含量进行测定，对其动态变化进行分析。结果表明：在插穗生根过程中，IAA、ZT含量均呈现先升高后降低而后缓慢升高的变化趋势；ABA含量呈现出在波动中下降的趋势，在愈伤组织形成期和不定根表达期达到最低值；GA3含量在整个生根过程中一直处于下降的趋势，仅在不定根表达期出现小幅度上升；相反，IAA/ABA比值基本处于在波动中上升的趋势。可见，较高含量的IAA、ZT及较高的IAA/ABA比值与扦插生根成正相关，而ABA和GA3与扦插生根成负相关。

关键词: 西安市, 西安市

Abstract: In order to establish efficient cutting technique system of Syringa microphylla, contents of 4 kinds of endogenous hormones (IAA、GA、ABA and ZR) in the process of adventitious root organogenesis were determined by hplc. The result showed that the contain of endogenous substance changed dynamically, in which the content of IAA and ZT increased first, then reduced, and finally rose slowly;but the content of ABA and GA3continued to decrease with fluctuation in the entire process. In the entire process of the adventitious root organogenesis, the tendency on the ratio of IAA/ABA went up continuously. The high activities of IAA, ZR and the ratios of IAA/ABA played a dominant role to promote the adventitious roots.