欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (21): 211-216.doi: 10.11924/j.issn.1000-6850.2013-3110

• 生物技术科学 • 上一篇    下一篇

利用SSR标记分析17个亚麻品种的遗传关系

张倩 姜恭好 杨学 曲志会 李杨 路颖 段海燕   

  • 收稿日期:2013-11-26 修回日期:2014-01-16 出版日期:2014-07-25 发布日期:2014-07-25

Analysis of Genetic Relationship Among 17 Flax Varieties by Using SSR Markers

  • Received:2013-11-26 Revised:2014-01-16 Online:2014-07-25 Published:2014-07-25

PDF (PC)

363

摘要： 本研究利用90对SSR引物对17个亚麻品种（品系）进行了遗传相似性的分析。SSR标记简便高效，特异性强，但目前在亚麻上的应用还比较少，本研究选择均匀分布在亚麻不同染色体上的90对SSR标记进行分析。结果表明：所有参试SSR引物均扩增出清晰稳定的多态性条带，在17个参试亚麻品种间共检测出170个不同的扩增条带。17个亚麻品种分成5个大群体，遗传距离最大的品种是‘9801-1-17’和‘阿卡塔’。第二组和第五组都只有一个品种，分别是‘A29’和‘9801-1-17’，它们与其他的品种间亲缘关系均较远。本研究表明SSR技术在亚麻遗传育种中有很大的应用前景，准确高效。遗传距离较远的几个品种可用于杂交育种和各种农艺性状的进一步遗传分析。

关键词: 综合模型, 综合模型

Abstract: The experiment took 90 SSR markers to analyze the genetic relationship among 17 flax cultivars. Although SSR markers have the advantages of high efficiency and specificity, which still got less application on flax currently. In this study, 90 SSR markers that evenly distributed on different flax chromosome were screened for polymorphism analysis. The result showed that clear and stable polymorphic bands were amplified for all of the tested SSR primers. A total of 170 different amplification bands were detected. 17 flax cultivars were divided into 5 groups, among them '9801-1-17' and 'Akata' had the largest genetic distance. The second group and the fifth group had only one species, which were 'A29' and '9801-1-17' respectively. They were far away from other cultivars in genetic relationship. This research showed that SSR technology should be widely utilized on the flax breeding for high accuracy and efficiency. The cultivars with distant genetic relationship can be used for hybridization breeding and further genetic analysis on agronomic traits.