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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (18): 277-282.doi: 10.11924/j.issn.1000-6850.2014-0031

所属专题： 油料作物

• 生物技术科学 • 上一篇    下一篇

2012年北方春大豆国家区试大豆品种纯度鉴定、分子ID构建及遗传多样性分析

何琳   

  • 收稿日期:2014-01-03 修回日期:2014-02-14 出版日期:2014-06-25 发布日期:2014-06-25
  • 基金资助:
    国家高技术研究发展计划“863计划”“高产优质抗逆大豆分子育种与品种创制”(2012AA101106)；现代农业产业技术体系大豆产业体系(CARS-04-02A)。

Purity Identification, Molecular ID Establishmentand Genetic Diversity Analysis of Soybeans Attending National Regional Test of NorthSpring Soybeanin 2012

  • Received:2014-01-03 Revised:2014-02-14 Online:2014-06-25 Published:2014-06-25

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摘要： 为了鉴定北方春大豆组国家区域试验大豆品种的纯度、构建参试大豆品种的分子ID及品种间遗传关系分析，从而有效地指导中国北方春大豆新品种的选育和推广。通过对参加2012年北方春大豆国家区试的94份大豆材料用分布在大豆基因组8个连锁群的13对SSR标记进行分析。结果表明：94份参试大豆品种纯度分布范围为53.85%~100%，平均纯度为99.02%；利用Satt231、Satt288、Satt160、Satt193和Sat-092这5对引物可以将94份参试大豆品种区分开，并获得唯一的分子ID；94个参试大豆品种间遗传相似系数为0~0.846，平均相似系数为0.2783，说明参试大豆品种间遗传差异性较大。通过SSR标记分析，可以有效地鉴定参试大豆品种的纯度、建立参试大豆品种的分子ID及实现品种间遗传关系分析。

Abstract: Purity identification, molecular ID and analysis of genetic relation of cultivars were completed for test cultivar in the national north spring soybean cultivar authorize. These work had great contribution to the cultivar breeding and spread. 13 SSR markers which distributed in 8 linkage groups of soybean were used to identify 94 cultivars belong to the tested varieties of national north spring soybean cultivar authorize in 2012. The results showed that the purity of these varieties ranged from 53.85% to 100%, and averaged 99.02%. Five SSR markers including Satt231, Satt288, Satt160, Satt193 and Sat-092, were used to identify the 94 soybean varieties, then a unique molecule ID was obtained for each variety. The genetic similar coefficient of 94 varieties ranged from 0 to 0.846, the average value was 0.2783, and the result indicated the genetic difference was huge in the 94 tested varieties. SSR analysis method was an effective method to identify the purity, construct molecular ID and complete analysis of genetic relation for the tested varieties.