关键词: EOF, EOF

Abstract: Trehalose glucose and polyol play an important role during insect diapause to suffer cold-hardness, and the volume of the chemical materials can reflect insect’s physiological status. This experiment measured the volume of glycogen trehalose glucose glycerin by the GC. The results showed that: the volume of trehalose rose firstly then fell down, reached the peak (273.5±19.34)μg/mL at the 30th day of cold treatment. Transferring the egg kept in cold for 150 days to the condition of 25℃, the volume of trehalose fell down sharply. The same as trehalose, glucose increased at the beginning of experiment, but slumped with cold stimulation, then the volume fluctuated slightly. After transferred in the 25℃, the volume of glucose maintained, which was different with trehalose. Inositol kept increasing through the experiment, mannitol increased slowly during pre-diapause, and fell slowly after 60 days’cold-stimulation, the volume of sorbitol waved sharply. From the changing trend of these polyol, we could conclude that it was not reliable to distinguish whether the egg entered or came out of the diapause only depending on the change of the chemical. During the experiment, the volume of inositol increased, the volumes of other kind of polyol soared first and then dropped down, which meant the eggs maintained high metabolism rate during diapause. The cold stimulation did not change the average eggs weight harshly, at the same time, the volumes of the chemical soared and droped, which meant huge amount of energy was transformed to metabolize. The volumes of three kinds of polyols increased then dropped, and increased again but dropped at the long run, that meant the polyols could transform into carbohydrate to supply energy.