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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 291-294.doi: 10.11924/j.issn.1000-6850.2014-1049

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甘草内生菌Z4菌株对灰霉病菌的作用机制初探

刘霞,马绥斌,李佳   

  1. 延安大学,延安大学生命科学学院,
  • 收稿日期:2014-04-12 修回日期:2014-04-12 接受日期:2014-07-01 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 刘霞
  • 基金资助:
    陕西省教育厅专项科研计划项目“陕北野生甘草内生菌的农用抑菌活性研究”(11JK0643)；陕西省科技攻关计划项目“陕北枣树重要虫害- 枣尺蠖的杀治技术研究”(2012K01-12)；陕西省教育厅省级重点实验室科研项目“陕北枣树流行病学调查及其重要虫害的防治技术研究”(12JS121)； 陕西省高水平大学建设专项资金资助项目“生物学学科建设”(2012SXTS06)；国家大学生创新训练计划项目“陕北枣树重要害虫的驯养及杀治研究” (201310719008)；国家大学生创新训练计划项目“新型抗昆虫蝎毒素多肽的筛选研究”(201310719005)；延安大学科研基金资助项目“天然产物活性成 分的分离与功能鉴定”(YD200653)

Preliminary Investigation of Mechanism of Action of Z4 Endophytic Strain Separated from Wild Liquorice for Botrytis cirerea

  • Received:2014-04-12 Revised:2014-04-12 Accepted:2014-07-01 Online:2014-10-15 Published:2014-10-15

摘要： 为探究甘草内生菌Z4 菌株的发酵上清液对灰霉病菌的抑菌机制，采用抑制菌丝生长速率法及抑制孢子萌发法，结合扫描电镜和透射电镜，观察Z4 菌株发酵上清液处理前后灰霉病菌菌丝形态结构的变化情况。采用盆栽试验测试活性物质在活体黄瓜内的传导性。通过扫描电镜、透射电镜及光镜观察发现，Z4 菌株的发酵上清液使番茄灰霉病菌菌丝扭曲变形、表面皱缩、部分菌丝断裂；细胞壁溶解破裂，细胞内液泡出现空腔；孢子萌发率低及芽管生长缓慢。盆栽黄瓜灌根和涂叶处理前后，黄瓜的病指分别由82.3 降至28.05 以及83.11 降至30.60，防治效果分别为63.12%和60.28%。表明Z4 菌株发酵上清液对灰霉病菌菌丝细胞的细胞壁具有破坏作用，且活性物质在活体黄瓜内有一定传导性。

关键词: 持水力性能评价, 持水力性能评价

Abstract: The study aims to investigate the antifungal mechanism of the supernatant fermentation from Z4 strain in vitro. The action mechanism of Z4 strain was surveyed by inhibiting the mycelial growth ratio and spore germination, combining scanning electronic microscope, transmission electronic microscope and potted plant. The results indicated that the fermentation extracts exhibited antifungal activities to mycelium growth of Botrytis cinerea. The fermentation extracts could inhibit the spore germination and the bud growth of Botrytis cinerea. Also the sample had a series of marked morphological and structural alterations of the mycelium under the light microscope and electronic microscope, such as prayer beads swollen of part of mycelium and malforming septa under scan electronic microscope, the cell wall dissolution and the cell liquid seepage under transmission electronic microscope. In addition, disease index of potted cucumber after treated by rootirrigation and spread on leaf were reduced from 82.3 to 28.05 and 83.11 to 30.60, respectively. Control efficiency of the fermentation products of Z4 strain against the Botrytis cinerea were 63.12% and 60.28%, respectively. The cytological changes above were resulted in the necrosis of mycelium cell wall. In addition, the conductivity of the sample displayed in internal of cucumber.