外源信号物质对肉苁蓉种子萌发与吸器形成POD、CAT活性及ROS含量的影响

doi:10.11924/j.issn.1000-6850.2014-1295

中国农学通报 ›› 2014, Vol. 30 ›› Issue (36): 128-132.doi: 10.11924/j.issn.1000-6850.2014-1295

所属专题：生物技术

外源信号物质对肉苁蓉种子萌发与吸器形成POD、CAT活性及ROS含量的影响

陈虞超,李苗,巩檑,宋玉霞

(宁夏农业生物技术重点实验室,银川 750002）

收稿日期:2014-05-05 修回日期:2014-10-23 接受日期:2014-11-19 出版日期:2015-03-20 发布日期:2015-03-20
通讯作者: 陈虞超
基金资助:
国家自然科学基金项目“肉苁蓉专性寄生外源信号物质作用研究(30960023)，“肉苁蓉属植物与寄主间水平基因转移研究”(31160066)；宁夏科技支撑计划项目“药用濒危植物肉苁蓉人工控制寄生技术研究”(2013ZYS148)；宁夏自然科学基金项目“肉苁蓉离体寄生体系的建立(NZ12262)。

Effects of Exogenous Signal Substances on POD, CAT Activity and ROS Content in Cistanche deserticola Y. C. Ma Seeds During Germination and Haustorium Formation

Chen Yuchao, Li Miao, Gong Lei, Song Yuxia

(Key Laboratory of Agricultural Biotechnology of Ningxia, Yinchuan 750002)

Received:2014-05-05 Revised:2014-10-23 Accepted:2014-11-19 Online:2015-03-20 Published:2015-03-20

摘要/Abstract

摘要： 利用外源信号物质氟草敏(norflurazon)和2,6-二甲氧基对苯醌(2,6-DMBQ)分别诱导肉苁蓉种子萌发与吸器形成，研究它们在此过程中对过氧化物酶(POD)、过氧化氢酶(CAT)活性及活性氧(ROS)含量的影响。结果表明：在诱导肉苁蓉种子萌发时，经norflurazon处理0~168 h后，种子中POD和CAT活性呈现显著升高的变化趋势，种子中ROS含量显著升高。在诱导肉苁蓉种子萌发体吸器形成时，经2,6-DMBQ处理0~72 h后，肉苁蓉种子萌发体中POD和CAT活性呈现显著升高的变化趋势，萌发体中ROS含量显著升高。表明在诱导肉苁蓉种子萌发与吸器形成过程中，外源信号物质norflurazon和2,6-DMBQ能影响POD、CAT活性及ROS含量的变化。

关键词: 化学计量特征, 化学计量特征

Abstract: The seed germination and haustorium formation of Cistanche deserticola were induced by norflurazon and 2,6-dimethoxy-p-benzoquinone (2,6-DMBQ) respectively. The effects of them on peroxidase(POD), catalase(CAT) activities and reactive oxygen species(ROS) content during seed germination and haustorium formation were studied. The results showed that POD, CAT activities and ROS content significantly increased during 0-168 h treated with norflurazon. POD, CAT activities and ROS content significantly increased during 0-72 h treated with 2,6-DMBQ. The results preliminarily confirmed that the exogenous signal substances, norflurazon and 2,6-DMBQ, effected POD, CAT activities and ROS content of C. deserticola during seed germination and haustorium formation.

陈虞超,李苗,巩檑,宋玉霞. 外源信号物质对肉苁蓉种子萌发与吸器形成POD、CAT活性及ROS含量的影响[J]. 中国农学通报, 2014, 30(36): 128-132.

Chen Yuchao,Li Miao,Gong Lei and Song Yuxia. Effects of Exogenous Signal Substances on POD, CAT Activity and ROS Content in Cistanche deserticola Y. C. Ma Seeds During Germination and Haustorium Formation[J]. Chinese Agricultural Science Bulletin, 2014, 30(36): 128-132.

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外源信号物质对肉苁蓉种子萌发与吸器形成POD、CAT活性及ROS含量的影响

Effects of Exogenous Signal Substances on POD, CAT Activity and ROS Content in Cistanche deserticola Y. C. Ma Seeds During Germination and Haustorium Formation

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