鸡血红素加氧酶-1（HO-1）和胆绿素还原酶A（BLVRA）基因表达量对蛋壳颜色的影响

doi:10.11924/j.issn.1000-6850.2014-1309

中国农学通报 ›› 2014, Vol. 30 ›› Issue (35): 64-68.doi: 10.11924/j.issn.1000-6850.2014-1309

所属专题：生物技术；畜牧兽医

鸡血红素加氧酶-1（HO-1）和胆绿素还原酶A（BLVRA）基因表达量对蛋壳颜色的影响

王晓庆,徐廷生

河南科技大学动物科技学院,河南科技大学动物科技学院

收稿日期:2014-05-06 修回日期:2014-05-06 接受日期:2014-08-18 出版日期:2015-03-18 发布日期:2015-03-18
通讯作者: 徐廷生
基金资助:
河南省重点科技攻关项目“绿壳蛋鸡分子育种技术的研究与应用”(82102130001)；洛阳市重点科技攻关项目“绿壳蛋鸡生态养殖模式的研究与示范”(0701040A)。

The Effects of the Expression Levels of H0-1 and BLVRA on the Color of Egg Shells

Received:2014-05-06 Revised:2014-05-06 Accepted:2014-08-18 Online:2015-03-18 Published:2015-03-18

摘要/Abstract

摘要： 研究旨在检测血红素加氧酶(HO-1)和胆绿素还原酶A(BLVRA)在卢氏鸡和固始鸡输卵管子宫部的表达量差异，探索绿壳蛋的形成机理。以24周龄产绿壳蛋卢氏鸡和产褐壳蛋固始鸡为素材，采集每只鸡输卵管子宫部，提取总RNA，经过RT-PCR反转录为cDNA，以与色素相关的血红素加氧酶-1(Heme Oxygenase，HO-1)和胆绿素还原酶A(BLVRA)为目的基因，选择GAPDH作为参照基因，通过实时荧光定量PCR，分析HO-1和BLVRA在不同品种鸡的机体内转录水平的差异。结果表明：卢氏鸡子宫部组织中HO-1的mRNA相对表达量显著高于固始鸡(P＜0.05)。固始鸡子宫部组织中BLVRA mRNA相对表达量极显著高于卢氏鸡(P＜0.01)。

关键词: 芽丛发生, 芽丛发生

Abstract: This study aims to detect the expression difference of HO-1 and BLVRA in, uterine part of Lushi chickens and Gushi chickens, thus discover the formation mechanism of green eggs. To conduct the experiment, Lushi chickens laying green eggs and Gushi chickens laying brown eggs, all of which were 24- week aged, were randomly selected and the uterus of each chicken was collected. Then the total RNA was extracted and later reversely transcribed into cDNA. The pigment-associated genes, HO-1 and BLVRA were chosen to be the target genes, and GAPDH the reference genes. Finally, the transcription levels of HO-1 and BLVRA in different breeds of chickens were analyzed using real- time fluorescent quantitation PCR. The results showed that the HO-1 mRNA expression level in the uterus of Lushi chickens was higher than that of Gushi chickens (P＜0.05), and that the BLVRA mRNA expression level in the uterus of Gushi chickens was significantly higher than that of Lushi chickens (P＜0.01).

王晓庆,徐廷生,雷雪芹,高灵照,肖赞奇. 鸡血红素加氧酶-1（HO-1）和胆绿素还原酶A（BLVRA）基因表达量对蛋壳颜色的影响[J]. 中国农学通报, 2014, 30(35): 64-68.

雷雪芹, 高灵照 and 肖赞奇. The Effects of the Expression Levels of H0-1 and BLVRA on the Color of Egg Shells[J]. Chinese Agricultural Science Bulletin, 2014, 30(35): 64-68.

参考文献

[1] Ito S, Tsudzuki M, Komort M, et al. Celadon:an eggshell color mutation in Japanese quail[J]. J Hered,1993,84(2) :145-147.
[2] Pool H K. Spectrophotometric identification of eggshell pigments and timing of superficial pigment deposition in the Japanese quail[J]. Proc Soc Exp Biol Med,1965,119:547-551.
[3] Kennedy G Y, Vevers H.G. A survey of avian eggshell pigments[J]. Comparative Biochemistry and Physiology,1976,55:117-123.
[4] 李纯华．蛋壳颜色形成的机理探讨[J]．四川畜牧兽医，2004，12：21-22．
[5] 许小志，何平．血红素加氧酶-1与氧化应激[J] ．中国当代儿科杂志，2009，11(2)：706-709．
[6] Ryter S W，Alam J，Choi A M，Heme oxygenase-1/carbon monoxide:from basic science to therapeutic applications[J]. Physiol Rev, 2006, 86(2)：583-650.
[7] 张雁林．血红素加氧酶-1的细胞保护作用研究进展[J]．环境与职业医学，2008，25(2)：197-201．
[8] Tenhunen R, Marver H S, Schmid R. The enzymatic conersion of heme to bilirubin by microsomal heme oxygenase[J]. Proc Natl Acad Sci USA, 1968, 61(2):748-755.
[9] 周爱儒．主编．生物化学[M]．第5 版，北京人民出版社，2000：378-379．
[10] Punnett R C，Bailey P G. Genetic studues in poultry:II. Inheritance of egg-colour and broodiness[J]. J Genet, 1920, 10:277-292.
[11] Benjamin E W. A study of selections for the size，shape，and color of hen’s eggs[J]. New York Agric Exp Station Memoir, 1920, 32:195-321.
[12] Kopec S. An experimental study of xenia in the domestic fow[J]. J Genet, 1926, 16:269-286.
[13] Hall G O. Egg shell color in crosses between white and brown-egg breeds[J]. Poult Sci, 1944, 23:259-265.
[14] Farnsworth G M，Nordskog A W. Breeding for egg quality 3.Genetic differences in shell characteristics and other egg quality factors[J]. Poult Sci, 1955, 34:16-26.
[15] Zhao R，Xu G Y，et al，A study on eggshell pigmentation：biliverdin in blue-shelled chickens[J]. Poult Sci, 2006, 85（3）:546-549.
[16] 岳慧洁，徐善金，韦小菲，等．鸭血红素加氧酶-1（HO-1）基因表达及对蛋壳颜色的影响[J]．中国兽医学报，2012，32（8）：1235-1238．
[17] 王哲鹏，王晓通，白激荣，等．血红素加氧酶-1在绿壳蛋蛋壳色素形成中的作用[C]．第十五次全国动物遗传育种学术讨论会论文集．陕西杨凌．2009：467．
[18] 李光奇，姚俊峰，曲鲁江，等．血红素加氧酶1(HO-1) mRNA在不同蛋壳颜色鸡群中的表达研究[C]．第十五次全国家禽学术讨论会论文集．广州：2011，92-95．
[19] Maines M D, Polevoda B V, et al. Human biliverdin IXα reductase is a zinc-metallo-protein. Characterization of purified and Escherichia coli expressed enzymes[J]. Eur J Biochem, 1996, 235（1-2）:372-381.

鸡血红素加氧酶-1（HO-1）和胆绿素还原酶A（BLVRA）基因表达量对蛋壳颜色的影响

The Effects of the Expression Levels of H0-1 and BLVRA on the Color of Egg Shells

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