欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (31): 295-299.doi: 10.11924/j.issn.1000-6850.2014-1310

所属专题： 玉米

• 植物保护 农药 • 上一篇    下一篇

甲草胺在玉米籽粒上的QuEChERS-HPLC残留分析方法探究

汪玉,高子登,张剑南,胡树文   

  1. 中国农业大学资源与环境学院环境科学与工程系,中国农业大学资源与环境学院环境工程与科学系,中国农业大学资源与环境学院环境科学与工程系,中国农业大学资源与环境学院环境科学与工程系
  • 收稿日期:2014-05-06 修回日期:2014-05-06 接受日期:2014-06-17 出版日期:2014-11-20 发布日期:2014-11-20
  • 通讯作者: 胡树文
  • 基金资助:
    农业部公益性行业专项“一次性施肥技术及推广示范”(201303103)；科技部“十二五”科技支撑计划“环境友好型高分子膜材料研发”(2011BAD11B02)

QuEChERS-HPLC Residue Analysis Method of Alachlor in Corn

  • Received:2014-05-06 Revised:2014-05-06 Accepted:2014-06-17 Online:2014-11-20 Published:2014-11-20

摘要： 建立了一种简单、快速、环保的甲草胺在玉米籽粒中的QuEChERS-HPLC残留分析方法。样品用极少量的乙腈提取，用N-丙基乙二胺（PSA）和石墨化炭黑（GCB）净化，高效液相色谱（紫外检测器）检测甲草胺浓度。色谱柱为C18反相柱(250 mm×4.6 mm×5.0μm)，流动相乙腈和水（70:30）溶液。对玉米中的甲草胺进行不同水平的添加回收率实验，在考察浓度范围内线性关系良好(R2≥0.994)；在0.2~10 mg/kg添加水平内，平均加标回收率为96.7%~113.8%，相对标准偏差（RSD）为3.2%~15.3%；检出限和定量限分别为0.015 mg/kg、0.05 mg/kg。该方法的精密度、灵敏度和准确度符合农药残留分析的要求。

关键词: 霜害温度指标, 霜害温度指标

Abstract: A simple, fast, and environmental-friendly determination method of alachlor residue in corn was established using QuEChERS- HPLC equipped with UV detector. Samples were extracted by a little acetonitrile, purified by N- propyl ethylenediamine (PSA) and graphitized carbon black (GCB). The concentration of alachlor was detected by HPLC-UV. The analytical column was C18 (250 mm×4.6 mm×5.0 μm) and the mobile phase was acetonitrile mixed with water (70:30 V/V). With a significant linear relation (R2≥0.994) of standard samples measured by this method, the average recovery rates of different alachlor doses (0.2-10 mg/kg) were 96.7%-113.8% and RSD were 3.2%-15.3%. The limit of detection and the limit of quantitation were 0.015 mg/kg and 0.05 mg/kg respectively. The accuracy, sensitivity and precision of this method can meet the requirement of pesticide residue analysis.