微波辅助提取燕麦总酚及其抗氧化能力评价

doi:10.11924/j.issn.1000-6850.2014-1827

中国农学通报 ›› 2015, Vol. 31 ›› Issue (7): 229-234.doi: 10.11924/j.issn.1000-6850.2014-1827

所属专题：小麦

微波辅助提取燕麦总酚及其抗氧化能力评价

武娇,任祎

山西农业大学,山西农业大学

收稿日期:2014-07-02 修回日期:2015-02-25 接受日期:2015-01-23 出版日期:2015-04-03 发布日期:2015-04-03
通讯作者: 任祎
基金资助:
国家科技支撑计划子课题(2012BAD34B05-18)；现代农业产业技术体系建设专项资金(CARS-08-A-1-6)；山西省基础研究项目(2012011031-2)；山西省自然基金(2012045)；国家燕麦产业体系(CARS-08-A-1)；国家自然科学基金(31201265)。

Determination of phenolic compounds and assessment of antioxidant activity of oat bran by microwave-assisted extraction

武娇 and 任祎

Received:2014-07-02 Revised:2015-02-25 Accepted:2015-01-23 Online:2015-04-03 Published:2015-04-03

摘要/Abstract

摘要： 本文对燕麦麸皮中总多酚进行微波提取，与热回流提取方法进行比较。探讨溶剂、料液比、微波功率、微波处理时间等对总酚得率的影响，并运用L9(34 )正交试验对微波加热提取燕麦麸皮总酚类物质的工艺条件进行了优化。结果表明，微波功率对总酚得率有显著影响，微波处理时间影响较小。最佳工艺是：75%乙醇，料液比（m/v）1:8，微波功率640 W，微波辐射时间15 min，此条件下提取燕麦麸皮总酚的提取得率为9.72 mg.g-1，高于传统的热回流提取多酚的得率6.67 mg.g-1。提取物的抗氧化能力用DPPH清除率表示，相当与同质量α-生育酚的90.4%。结论：利用微波加热提取燕麦麸皮中的多酚比传统的热回流方法效率高，提取物具有较强的抗氧化活性。

关键词: 三七根腐病、挥发物、浸提液、恶疫霉菌、腐皮镰孢菌, 三七根腐病、挥发物、浸提液、恶疫霉菌、腐皮镰孢菌

Abstract: In order to set up a efficient method for extracting total phenolic compounds from oat bran, and reducing enviormental pollution, the Microwave-assisted extraction (MAE) was applied in the extraction and compared with traditional solvent extraction (TSE) as well. Effects of various conditions including the solvent, solvent to material ratio, microwave power and irradiation time on extraction yield of phenolics were investigated, the conditions was optimized by orthogonal design as well. The results showed that the optimal parameters for MAE were 75% of ethanol, 640 W of microwave power, 1:8 g/mL of solid-liquid ratio and 15 min irradiation time; under such conditions, 9.72 mg/g phenolic extraction yield was achieved with MAE, which was higher than 6.67 mg/g with TSE. The vitro antioxidant activity of the extract was evaluated by using DPPH assay. The extract with MAE had strong capacity of scavenging DPPH free radical, which was 90.4% as large as that of α-tocopherol.

武娇,任祎. 微波辅助提取燕麦总酚及其抗氧化能力评价[J]. 中国农学通报, 2015, 31(7): 229-234.

武娇 and 任祎. Determination of phenolic compounds and assessment of antioxidant activity of oat bran by microwave-assisted extraction[J]. Chinese Agricultural Science Bulletin, 2015, 31(7): 229-234.

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微波辅助提取燕麦总酚及其抗氧化能力评价

Determination of phenolic compounds and assessment of antioxidant activity of oat bran by microwave-assisted extraction

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