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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (31): 280-284.doi: 10.11924/j.issn.1000-6850.2014-2151

• 植物保护 农药 • 上一篇    下一篇

线柱苣苔无菌萌发及快速繁殖研究

姚绍嫦,蓝祖栽,凌征柱   

  1. 广西药用植物园,广西壮族自治区药用植物园,广西壮族自治区药用植物园
  • 收稿日期:2014-08-07 修回日期:2014-08-07 接受日期:2014-09-30 出版日期:2014-11-20 发布日期:2014-11-20
  • 通讯作者: 姚绍嫦
  • 基金资助:
    广西卫生厅科研课题“药用唇柱苣苔等六种苦苣苔科药用植物离体种质保存技术研究”(Z2011156)

Germination and Rapid Propagation from Seed of Rhynchotechum obovatum (Griff.) Burtt

  • Received:2014-08-07 Revised:2014-08-07 Accepted:2014-09-30 Online:2014-11-20 Published:2014-11-20

摘要： 为了通过组织培养方法建立线柱苣苔高效再生体系，以线柱苣苔种子为外植体，在MS培养基中添加6-BA与NAA不同浓度激素配比，筛选初代培养、继代增殖与生根培养等各阶段最适的培养基。结果表明，用0.1% HgCl2溶液对线柱苣苔蒴果与种子灭菌10 min可有效抑菌。种子萌发及不定芽诱导的最适培养基为MS+1.0 mg/L 6-BA+0.2 mg/L NAA。最佳的继代增殖培养基为MS+0.5 mg/L 6-BA+0.2 mg/L NAA，生根培养基为1/2 MS+0.2 mg/L NAA。利用本试验所得出的结果，增殖系数为13.5/20天，可实现线柱苣苔种苗的快速繁殖，为种质资源的保护和可持续利用提供技术指导。

关键词: 杭州, 杭州

Abstract: The research aims to develop rapid propagation system from seed of Rhynchotechum obovatum (Griff.) Burtt via tissue culture. The seed of Rhynchotechum obovatum acting as explants were germinated and cultured on MS supplemented with different concentrations of hormone in order to select the optimum medium for different phases. The results showed that, the seeds and capsules were efficiently sterilized by 0.1% HgCl2 for 10 min. The optimum medium for germination and buds induction was MS 1.0 mg/L 6-BA 0.2 mg/L NAA. The suitable multiplication medium was MS 0.5 mg/L 6-BA 0.2 mg/L NAA and the suitable rooting medium was 1/2 MS 0.2 mg/L NAA. Using the methods obtained in this experiment, in vitro rapid propagation of Rhynchotechum obovatum could be achieved at the multiplication rate of 13.5 per 20 days, which could develop more seedlings in short time and provide guidance for the conservation and sustainable development of the resource.