陈年普洱茶香气成分及清除DPPH自由基活性研究

doi:10.11924/j.issn.1000-6850.2014-2153

中国农学通报 ›› 2015, Vol. 31 ›› Issue (4): 274-279.doi: 10.11924/j.issn.1000-6850.2014-2153

所属专题：园艺

陈年普洱茶香气成分及清除DPPH自由基活性研究

陈梅春¹,张海峰²,潘志针¹,陈峥¹,朱育菁¹,车建美¹,史怀¹,刘波¹

(¹福建省农业科学院农业生物资源研究所,福州 350003；²福建省农业科学院科研管理处,福州 350003）

收稿日期:2014-08-07 修回日期:2015-02-03 接受日期:2014-11-24 出版日期:2015-03-20 发布日期:2015-03-20
通讯作者: 刘波
基金资助:
国家“948”项目“高效新型微生物资源引进与创新”(2011-G25)。

Analysis of Aroma Components and DPPH· Scavenging Activity of Aged Pu-erh Tea

Chen Meichun¹, Zhang Haifeng², Pan Zhizhen¹, Chen Zheng¹, Zhu Yujing¹, Che Jianmei¹, Shi Huai¹, Liu Bo¹

(¹Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003;²Fujian Academy of Agricultural Sciences, Fuzhou 350003)

Received:2014-08-07 Revised:2015-02-03 Accepted:2014-11-24 Online:2015-03-20 Published:2015-03-20

摘要/Abstract

摘要： 为研究陈年普洱茶的特征风味成分及生物活性，采用顶空-固相微萃取-气质联用法测定了3种陈年普洱茶中挥发性物质，结合质谱和保留指数共鉴定出57种化合物，其中甲氧基酚类、醇类及酮类化合物为主要香气成分。同时采用比色法测得3种普洱茶的沸水提取物的茶多酚含量分别为(41.91±1.98)、(43.84±2.56)及(41.28±2.57) mg GAE/(g·DW)，并对3种普洱茶的1,1-二苯基苦基苯肼(DPPH·)自由基清除效果进行研究。结果表明3种普洱茶均具有较好的清除DPPH自由基能力，且呈剂量依赖性，测得3种普洱茶提取物清除DPPH·自由基的IC₅₀分别为(129.03±0.25)、(139.47±1.58)及 (152.57±3.34) mg GAE/L。

关键词: 沙漠边缘不同植被土壤理化性质, 沙漠边缘不同植被土壤理化性质

Abstract: The aim of this study was to investigate the flavor characteristics and bioactivity of aged Pu-erh tea. Volatile compounds from aged Pu-erh tea were measured using solid-phase micro-extraction (SPME) and gas chromatography-mass spectrometry (GC-MS). Results showed that a total of 57 volatile compounds were identified by matching EI mass spectra and retention indices with the literature data. Alcohols, methoxy-phenolic and ketones were the three major aromatic groups. The total phenolic contents of three types of Pu-erh tea were (41.91±1.98), (43.84±2.56) and (41.28±2.57) milligrams of gallic acid equivalent per gram of dried weight [mg GAE/(g·DW)]. The effects of Pu-erh tea extracts on the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) scavenging activity were evaluated. The results showed that all types Pu-erh tea extracts exhibited the relative strong DPPH· scavenging activity and in dosage-dependent modes. The concentration of sample required for 50% inhibition of DPPH· (IC₅₀) were (129.03±0.25), (139.47±1.58) and (152.57±3.34) mg GAE/L, respectively.

陈梅春,张海峰,潘志针,陈峥,朱育菁,车建美,史怀,刘波. 陈年普洱茶香气成分及清除DPPH自由基活性研究[J]. 中国农学通报, 2015, 31(4): 274-279.

Chen Meichun,Zhang Haifeng,Pan Zhizhen,Chen Zheng,Zhu Yujing,Che Jianmei,Shi Huai and Liu Bo. Analysis of Aroma Components and DPPH· Scavenging Activity of Aged Pu-erh Tea[J]. Chinese Agricultural Science Bulletin, 2015, 31(4): 274-279.

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陈年普洱茶香气成分及清除DPPH自由基活性研究

Analysis of Aroma Components and DPPH· Scavenging Activity of Aged Pu-erh Tea

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