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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (11): 98-102.doi: 10.11924/j.issn.1000-6850.2014-2281

• 水产 渔业 • 上一篇    下一篇

应用PCR-DGGE技术分析福寿螺胃肠道菌群

程天印,曲凌玄,王晓君   

  1. 湖南农业大学,湖南农业大学,湖南农业大学
  • 收稿日期:2014-08-21 修回日期:2015-03-27 接受日期:2014-11-18 出版日期:2015-05-06 发布日期:2015-05-06
  • 通讯作者: 程天印
  • 基金资助:
    湖南省财政基金“入侵生物福寿螺的综合防治研究”（湘财农指(2009)55号）

The Flora of the Pomacea canaliculata Gastric and Intestinal Contents with PCR-DGGE

  • Received:2014-08-21 Revised:2015-03-27 Accepted:2014-11-18 Online:2015-05-06 Published:2015-05-06

摘要： 为了探究福寿螺消化纤维素的生理机制，本研究采用PCR-DGGE技术分析了福寿螺胃肠内容物的菌群结构。无菌条件下采集福寿螺胃、肠内容物，提取细菌总DNA；以通用引物扩增细菌16S rDNA V3区；DGGE电泳，回收、克隆、测定DGGE优势条带。结果表明，雌雄福寿螺胃肠菌群结构相同，均为22种细菌；10个明显条带的序列分别与气单胞菌属（Aeromonas sp）、希瓦氏菌属（Shewanella sp）、粘质沙雷菌（Serratia marcescens）、肠杆菌属（Enterobacter）、铁单胞菌属（Ferrimonas sp）和5个不可培养的细菌的16S rDNA V3区序列高度相似。分离到2株细菌，其中1株为腐败希瓦氏菌。

关键词: 钙离子, 钙离子

Abstract: In order to explore on the digestive physiological mechanism of fiber, the florae of the P.canaliculata gastric and intestinal contents ware determined with PCR-DGGE. the analysis process were as followes: Collecting the gastric and intestinal contents in sterile conditions, extracting the bacterial total genomic DNA, amplifying the 16S rDNA V3 with universal primers, DGGE electrophoresis; recycling, cloning and sequencing the selected bands. The results show that the florae of the male and female P. canaliculata gastric and intestinal contents were the same, containing 22 bacteria; the sequences of the ten distinct bands are highly similar with Aeromonas sp, Shewanella sp, Serratia marcescens, Enterobacter, Ferrimonas sp and other uncultured bacteria. two kinds of bacteria were separated, one of them is Shewanella putrefaciens.