阿特拉津残留对黑土农田中AM真菌多样性的影响

doi:10.11924/j.issn.1000-6850.2014-2357

中国农学通报 ›› 2015, Vol. 31 ›› Issue (2): 174-180.doi: 10.11924/j.issn.1000-6850.2014-2357

阿特拉津残留对黑土农田中AM真菌多样性的影响

王辰¹,宋福强¹,孔祥仕²,李季泽¹

(¹黑龙江大学生命科学学院,哈尔滨 150080；²南京大学生命科学学院,南京 210093）

收稿日期:2014-08-28 修回日期:2015-01-15 接受日期:2014-09-29 出版日期:2015-03-19 发布日期:2015-03-19
通讯作者: 宋福强
基金资助:
国家自然科学基金面上项目“丛枝菌根对阿特拉津污染土壤修复机制研究”(31270535)；黑龙江省自然科学基金重点项目“菌根生物技术修复阿特拉津污染土壤作用机制研究”(ZD201206)。

Effects of Atrazine Residues on the Diversity of AM Fungi in Black Soil Farmland

Wang Chen¹, Song Fuqiang¹, Kong Xiangshi², Li Jize¹

(¹Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080;²School of Life Science, Nanjing University, Nanjing 210093)

Received:2014-08-28 Revised:2015-01-15 Accepted:2014-09-29 Online:2015-03-19 Published:2015-03-19

摘要/Abstract

摘要： 为了揭示阿特拉津残留对黑土农田中AM真菌多样性的影响，利用湿筛倾析-蔗糖离心法对哈尔滨、齐齐哈尔和黑河地区多年连续施加阿特拉津的农田土壤中的AM真菌进行分离鉴定。结果表明，共计分离出4属47种AM真菌，包括无梗囊霉属Acaulospora 16种、球囊霉属Glomus 27种、盾巨孢囊霉属Scutellospora 3种和巨孢囊霉属 Gigaspora 1种。阿特拉津残留量较低时对AM真菌孢子密度和物种丰度影响不显著，当阿特拉津残留量高于0.28 mg/kg时，AM真菌孢子密度和物种丰富度与阿特拉津残留量呈显著负相关。Glomus属和Acaulospora属为3个地区的主要优势菌属。

关键词: 基因组改组技术, 基因组改组技术

Abstract: In order to reveal the effects of atrazine residues on the diversity of AM fungi in black soil farmland, wet-sieving and decanting-sucrose centrifugation method was employed to isolate AM spores from black soil farmland of Harbin, Qiqihar and Heihe where atrazine was applied continuously for many years, and then the isolated spores were identified. Totally we isolated 47 AM fungal species belonging to 4 genera, including 16 species that belonged to Acaulospora, 27 to Glomus, 3 to Scutellospora, and 1 to Gigaspora. Besides, we found that low concentration of atrazine residues showed no significant effects on AM fungi spore densities and species richness, while when the concentration of atrazine residues was higher than 0.28 mg/kg, AM spore density and species richness of AM fungi significantly and negatively correlated with the residues level of atrazine. In addition, Acaulospora and Glomus were found to be the dominant genus of the three regions.

王辰,宋福强,孔祥仕,李季泽. 阿特拉津残留对黑土农田中AM真菌多样性的影响[J]. 中国农学通报, 2015, 31(2): 174-180.

Wang Chen,Song Fuqiang,Kong Xiangshi and Li Jize. Effects of Atrazine Residues on the Diversity of AM Fungi in Black Soil Farmland[J]. Chinese Agricultural Science Bulletin, 2015, 31(2): 174-180.

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阿特拉津残留对黑土农田中AM真菌多样性的影响

Effects of Atrazine Residues on the Diversity of AM Fungi in Black Soil Farmland

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