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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (19): 149-153.doi: 10.11924/j.issn.1000-6850.casb14120096

• 林学 园艺 园林 • 上一篇    下一篇

柴胡根部中氨基酸含量的测定及分析

马艳芝   

  1. 唐山师范学院 生命科学系
  • 收稿日期:2014-12-15 修回日期:2015-05-11 接受日期:2015-05-22 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 马艳芝
  • 基金资助:
    国家科技部子课题“板蓝根、北柴胡规范化种植基地及其SOP优化升级研究”(2011BAI07B05-4)；河北省科技厅项目“菘蓝抗根腐病突变体的筛选及抗性基因的初步分离鉴定”(10965511D)；唐山市科技重大项目“唐山地区道地药材选育及其应用示范”(12130203A)。

Content Determination and Analysis of Amino Acid in Radix Bupleuri Root

  • Received:2014-12-15 Revised:2015-05-11 Accepted:2015-05-22 Online:2015-07-28 Published:2015-07-28

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摘要： 旨在了解柴胡的营养价值与保健药用功效。以11种柴胡的干燥根为试验材料，利用L-8900型氨基酸分析仪测定其氨基酸的组成及含量并进行分析。结果表明，供试材料含有17种氨基酸，种类较齐全。其中包含人体必需氨基酸7种，儿童必需氨基酸2种，各类味觉氨基酸等，色氨酸由于在酸水解过程中被破坏，未进行测定。这11种柴胡的氨基酸的E/N值在0.291~0.510；E/T值在22.5%~33.8%，接近理想蛋白质的要求；RAA及RC的数值大都接近于1，SRC的数值都在80以上，接近100。根据FAO/WHO修订的人体必需氨基酸含量模式谱，这11种柴胡根部必须氨基酸占氨基酸的比例大部分符合该谱，只有亮氨酸、苯丙氨酸+酪氨酸中度缺乏。

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Abstract: The aim was to study the nutrient value and health care medicinal effect of Radix Bupleuri. The amino acid content and composition of dry roots of 11 kinds of Radix Bupleuri were tested by L-8900 type amino acid analyzer and analyzed in this paper. The result indicated that there were 17 kinds of amino acids in the tested materials, including 7 kinds of essential amino acids for human body and 2 kinds of essential amino acids for children as well as many kinds of taste amino acids, etc. Tryptophan was not detected because it was destroyed in the process of hydrolysis. The E/N value of amino acid in 11 kinds of radix bupleuri was 0.291- 0.510; the E/T value of amino acid was 22.5%-33.8%, the RAA and RC were numerically close to 1, and the value of SRC was above 80, which was close to 100. The proportions of the essential amino acid to the total amino acid in the roots of 11 kinds of Radix Bupleuri were mostly in accordance with the FAO/WHO amino acid pattern spectrum. But leucine and phenylalanine+tyrosine were moderately short.