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中国农学通报 ›› 2015, Vol. 31 ›› Issue (19): 144-148.doi: 10.11924/j.issn.1000-6850.casb14110175

• 林学 园艺 园林 • 上一篇    下一篇

北虫草多糖提取工艺优化及抗氧化作用研究

元玲刚,常明昌,刘靖宇,刘勇男   

  1. 山西农业大学,山西农业大学,山西省晋中市山西农业大学,山西省晋中市山西农业大学
  • 收稿日期:2014-11-30 修回日期:2015-02-12 接受日期:2015-03-03 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 常明昌
  • 基金资助:
    山西省煤基重点科技攻关项目“设施食用菌高效碳循环研究与示范”(FT20140301);山西省科技攻关项目“山西省北冬虫夏草营养与栽培的研究开发”(20090311047)。

Study on Extraction and Antioxidation of Cordyceps Polysaccharide

  • Received:2014-11-30 Revised:2015-02-12 Accepted:2015-03-03 Online:2015-07-28 Published:2015-07-28

摘要: 为了研究北虫草多糖的最佳提取工艺以及抗氧化功能,以北虫草(Cordycepsmilitaris)子实体为试验材料,通过正交L9(34)试验探讨提取北虫草多糖的最佳工艺,并对北虫草多糖总还原力、DPPH清除能力、抑菌能力等进行了测定。结果表明,超声波辅助热水浸提法提取北虫草多糖的最佳工艺参数为:超声功率105W、超声时间40min、料水比1:25、热水浸提时间40min、热水浸提温度70℃,在此条件下的多糖得率为2.6602%。当北虫草浓度为2.4mg/mL时,还原力最高;0.45mg/mL时,DPPH清除能力最强。

关键词: 做形, 做形, 黄檗玉针茶, 品质

Abstract: In order to study the best extraction technology of Cordyceps polysaccharide of Cordyceps militaris and its antioxidant function, orthogonal testing [L9(34)] was used to optimize extraction condition for extracting polysaccharide from fruiting body of Cordyceps militaris. Total reducing power, scavenging activity of DPPH and antibacterial capacity of Cordyceps polysaccharide were measured. The results showed that the optimal parameters of ultrasound-assisted hot water extracting Cordyceps polysaccharide were ultrasonic power of 105 W, ultrasonic time of 40 minutes, liquid-solid ratio of 1:25, hot water extraction time of 40 minutes, hot water extraction temperature at 70℃. Yield of polysaccharide was 2.6602% under the optimized condition. Reducing power was the highest when the concentration of Cordyceps militaris was 2.4 mg/mL. DPPH scavenging power was the strongest when the concentration of Cordyceps militaris was 0.45 mg/mL.