甜菜细胞质雄性不育相关基因的克隆表达及CMS的相关性研究

doi:10.11924/j.issn.1000-6850.casb14120099

中国农学通报 ›› 2015, Vol. 31 ›› Issue (11): 137-142.doi: 10.11924/j.issn.1000-6850.casb14120099

所属专题：生物技术；园艺

甜菜细胞质雄性不育相关基因的克隆表达及CMS的相关性研究

乌日汉,斯钦巴特尔老师,伊六喜,田自华

内蒙古农业大学农学院植物学专业植物发育生理教研室,内蒙古农牧业科学院,内蒙古农牧业科学院,内蒙古农业大学农学院植物学专业植物发育生理教研室

收稿日期:2014-12-16 修回日期:2015-01-05 接受日期:2015-01-05 出版日期:2015-05-06 发布日期:2015-05-06
通讯作者: 田自华
基金资助:
国家甜菜产业技术体系重点项目(CARS-210402)。

Cloning and Expression of Cytoplasmic Male Sterility Associated Genes in Sugar Beet and Its Relationship to CMS

Received:2014-12-16 Revised:2015-01-05 Accepted:2015-01-05 Online:2015-05-06 Published:2015-05-06

摘要/Abstract

摘要： 已有的研究表明，线粒体atpA基因和orf187片段与作物雄性不育关系密切。为了研究atpA基因和orf187片段与甜菜细胞质雄性不育之间的关系，采用同源序列法分离克隆了甜菜体内atpA和orf187序列；序列分析揭示这2个片段序列在不育系和相应保持系间存在差异，进一步利用半定量RT-PCR和Real-time PCR技术分析了两系的atpA基因和orf187片段的表达情况。结果表明，与保持系相比，atpA在不育系中发生了碱基的置换及插入；orf187在不育系中缺失6个碱基，而在保持系中多了6 bp的重复序列；基因表达分析也显示，不育系atpA在花蕾发育的前2个时期表达量明显低于相应的保持系，而在大花蕾期其表达量又高于相应的保持系；orf187在不育系小花蕾时期表达量明显高于相应的保持系，随着花蕾的发育，该序列在不育系中的表达逐渐减弱并明显低于对应保持系中的表达量。推测atpA和orf187的结构变异及异常表达与甜菜细胞质雄性不育有着密切关系。

关键词: 宁武县, 宁武县

Abstract: Previous studies indicated that Mitochondrial atpA gene and orf187 fragment were closely related to male sterility in Crops. In order to research the relationship of atpA gene with orf187 fragment and cytoplasmic male sterility in sugarbeet, atpA gene and orf187 fragment in sugarbeet were isolated and cloned using homology- based technique. The sequence differences in sugarbeet sterile line and its corresponding maintainer line were defined according to sequence analysis, expression analysis of atpA gene and orf187 fragment was studied by further use of the semi- quantitative RT- PCR and Real- time PCR. The results indicated that compared with maintainer line, sterile line atpA had the replacement of bases and insertion; 6 base pairs were missing in sterile line orf187. The expression analysis demonstrated that expression of atpA in sugarbeet sterile line was significantly lower than that in maintainer line besides the big flower bud period, while expression of orf187 in sugarbeet sterile line was apparently higher than that in maintainer line besides the big flower bud period. These results indicated that structural variation and abnormal expression pattern of atpA and orf187 has a close relationship with sugarbeet cytoplasmic male sterility.

乌日汉,斯钦巴特尔老师,伊六喜,田自华. 甜菜细胞质雄性不育相关基因的克隆表达及CMS的相关性研究[J]. 中国农学通报, 2015, 31(11): 137-142.

参考文献

[1]胡毓琪等.华北地区节水型农业技术.北京：中国农业科技出版社，1995：85-138.
[2]北京农业大学主编.植物生理学.北京：农业出版[1] 胡毓琪.华北地区节水型农业技术[M].北京:中国农业科技出版社,1995:85-138.
[2] Levings C S, Pring D R. Restriction endonuelease analysis of mitochondria DNA from normal and texas cytoplasmic male sterile maize[J]. Science,1976(193):158-160.
[3] Dewey R E, Levings CS 3 R D, Timothy D H. Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male - sterile cytoplasm[J]. Cell,1986(44):439-449.
[4] Hanson M R, Bentolia S. Interactions of mitochondrial and nuclear genes that affect male gametophyte development[J]. The Plant Cell, 2004(16):S154-S169.
[5] 黄思齐,陈艳翠,李建军,等.红麻雄性不育相关基因 atp1的表达初步分析[J].中国麻业科学,2013,35(5):1671-3532.
[6] 张晓,张锐,史计,等.陆地棉胞质雄性不育系与保持系线粒体 SCAR分子标记[C].中国棉花学会2011年年会论文汇编,2011.
[7] 段继强,李建勇,杜光辉,等.苎麻线粒体基因 coxII和 atpA与细胞质雄性不育相关性分析[J].中国农业科学,2009,42(2):434-445.
[8] 李莉,杨波.甜菜雄性不育杂种优势利用及存在问题的解决[J].中国甜菜糖业,2009(1):38-39.
[9] 施真.甜菜线粒体育性相关基因的对比及 atp6基因的 RNA编辑研究[D].哈尔滨:哈尔滨工业大学,2012.
[10] 缪颖.植物雄性不育基因的研究进展[J].植物学报,2000,17(1):1- 10.
[11] 李大东.水稻线粒体 atpA基因的克隆及其与细胞质雄性不育的关系[D].北京:中国科学院遗传研究所,1990.
[12] 崔明辉,吴建勇,戚延香,等.植物细胞质雄性不育相关基因及其分子标记研究[J].分子植物育种,2010,8(5):1033-1042.
[13] 马继鹏.辣椒细胞质雄性不育相关基因的克隆及表达研究[D].杨凌:西北农林科技大学,2007:31-32.
[14] 张明方,杨景华,喻景权.榨菜细胞质雄性不育系及其保持系 atpA 基因组结构差异[A].蔬菜分子育种研讨会论文集,2004.
[15] 赵艳红.植物 CMS线粒体相关开放阅读框(ORFs)研究进展[D].南宁:广西农业科学院经济作物研究所,2011.
[16] Dewey R E, Timothy D H, Levings C S. Chimeric mitochondrial genes expressed in the C male- sterile cytoplasm of maize[J]. Current Genetics,1991(20):475-482.
[17] 张鸿,陈祖玉,李阳生,等.马协型水稻细胞质雄性不育系线粒体基因组的变异与功能的研究[J].武汉植物学研究,2007,25(3):313- 315.
[18] Horn R, Kohler R H, Zetsche K. A mitochondrial 16kDa protein is associated with cytoplasmic male sterility in sunflower[J]. Plant Molecular Biology,1991,17(1):29-36.
[19] Mart C J, Moneger F, Leaver C J. Cell-specific regulation of gene expression in mitochondria during anther development in sunflower[J]. The Plant Cell,1994,6(6):811-825.
[20] Edqbist J, Bergman P. Nulcear identity specifies transcriptional initiation in plant mitochondria[J]. Plant Molecular Biology,2002,49 (1):59-68.
[21] 巩养仓.棉花细胞质雄性不育相关线粒体基因筛选[D].北京:中国农业学院,2008.
社，1980.
[3]赵微平.作物生理.北京：农业出版社，1982.
[4]Levings C S, Pring D R. Restriction endonuelease analysis of mitochondria DNA from normal and texas cytoplasmic male sterile maize[J]. Science, 1976,193:158-160.
[5] McCollum G D. Occurrence of petaloid stamens in wild carrot(Daucus carota) from Sweden[J]. Economic Botany,1966, 20(4):361-367.
[6]于春霞,梁毅,李景富.胡萝卜雄性不育性的研究及利用[J].北方园艺,2006,(4):58-60.
[7]周玮,刘齐元,雄性不育烟草atp6基因育性相关生物信息学分析.核农学报2012,26（1）：0054-0059.
[8]Mikami T Sugiura M Kinoshita T(1984) Moecular heterogeneity in mitochondrial and choroplast DNAs from normal and male sterile cytoplasms in sugar beet Curr. Genet. 8:319-322
[9]Deway R E Levings C S III, Timotly D H,1986. Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the texas male-sterile cytoplasm. Cell,14;44(3):439-49.
[10]缪颖,植物雄性不育基因的研究进展〔J〕.植物学报,2000,17(1):1-10.
[11]李大东,水稻线粒体atpA基因的克隆及其与细胞质雄性不育的关系.1990.
[12]Akagi H, Sakamoto M & Shinjyo C, et al. A unique sequence located downstream from the rice mitochondrial atp6 gene may cause male sterility[J]. Curr Genet, 1994,25(1):52-58.
[13]马继鹏.辣椒细胞质雄性不育相关基因的克隆及表达研究[D].杨凌:西北农林科技大学,2007:31-32.
[14]路铁刚,丁毅.分子遗传学[M].北京:高等教育出版社,2008:83-84.
[15]周晓娟,赵瑞,洋葱细胞质雄性不育研究进展.北方园艺2012（17）：190-194.
[16]巩养仓等.棉花细胞质雄性不育相关线粒体基因筛选.中国农业学院,2008.
[17]段继强等.苎麻线粒体基因coxII和atpA与细胞质雄性不育相关性分析.中国农业科学，2009,42（2）：434-445.
[18]Masayuki P. Yamamoto? Tomohiko Kubo Tetsuo Mikami,The 5'-leader sequence of sugar beet mitochondrial atp6 encodes a novel polypeptide that is characteristic of Owen cytoplasmic male sterility.Mol Gen Genomics (2005)273:342-349.
[19]汪静,曹墨菊,玉米不育系及其保持系线粒体atp6基因转录本编辑位点研究.ISSN 0253-9772，2007,29（6）：731-737.
[20]韩艳芬,张龙雨,黏类小麦细胞质雄性不育线粒体atp6基因转录本编辑位点.作物学报2010, 36(12): 2179-2184；ISSN 0496-3490;2010,36（12）.
[21]李镭,郑用琏,玉米S组CMS胞质与正常胞质育性相关基因基于SSP-PCR的遗传多型性研究.作物学报2000,26(6).
[22]吴海涛,马蓉丽,刘洪炯,洋葱细胞质雄性不育系选育研究进展[J].园艺学报2009,36(2):297-302.
[23]Kaul,1988;Hanson & Folkerts,1992;Dragoeva & Atanassov,2001.
[24]Mineo Senda,Tetsuo Mikami,Toshiro Kinoshita.the sugar beet mittochondrial gene for the ATPase alpha-subunit:sequence,transcription and rearrangements in cytoplasmic male-sterile plants.Curr Genet(1993)24:164-170.

甜菜细胞质雄性不育相关基因的克隆表达及CMS的相关性研究

Cloning and Expression of Cytoplasmic Male Sterility Associated Genes in Sugar Beet and Its Relationship to CMS

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